高浓度葡萄糖对胰岛细胞凋亡及凋亡相关基因的影响  

Study on the Effects of High Concentration Glucose on Islet Cell Apoptosis and Apoptosis Related Gene

作　　者：何庆[1] 刘铭[2] 苏京[3] 王保平[3] 王晶[3] 陈克勤[3] 尹潍[1] 

机构地区：[1]天津医科大学总医院内分泌科,300052 [2]天津医科大学总医院老年病研究室,300052 [3]丹麦诺和诺德中国研究发展中心

出　　处：《天津医药》2006年第3期176-179,共4页Tianjin Medical Journal

基　　金：天津市自然科学基金资助课题(项目编号:983702411;043607911)

摘　　要：目的:观察高浓度葡萄糖体外对胰岛细胞凋亡及凋亡相关基因表达的影响,探讨葡萄糖毒性及其分子机制。方法:应用TUNEL法检测高浓度葡萄糖培养后大鼠胰岛细胞和小鼠βTc3细胞凋亡百分率;应用定量RT-PCR(QRT-PCR)检测培养后大鼠胰岛细胞和小鼠βTc3细胞bcl-2和baxmRNA的表达;应用定量RT-PCR检测低剂量链脲佐菌素糖尿病大鼠胰岛bcl-2和baxmRNA的表达。结果:高浓度葡萄糖使原代培养的大鼠胰岛细胞和βTc-3的凋亡细胞比例明显增加;胰岛细胞凋亡过程中,诱导凋亡基因bax的mRNA表达水平明显升高,抵抗凋亡基因bcl-2mRNA表达水平明显下降,致使bcl-2/bax比率明显降低;对低剂量链脲佐菌素糖尿病大鼠胰岛凋亡相关基因QRT-PCR检测也显示同样的结果。结论:高浓度葡萄糖可能通过诱导胰岛细胞凋亡增加而加重糖尿病,其中bcl-2/baxmRNA表达比率变化可能起重要作用。Objective： To study the effects of high concentration glucose on islet cell apoptosis and apoptosis related genes, and discuss the glucose toxicity and its molecular mechanism. Methods： Apoptosis percentages of primary rat islet cells and βTc3 cells cultured with high concentration glucose medium were measured by TUNEL method, mRNA expressions of bcl-2 and bax of cultured.rat islet cells and mouse βTc3 cells were detected by QRT-PCR. mRNA expressions of bcl-2 and bax of low dosage streptozotocin （STZ） diabetic rat islet cells were measured by QRT-PCR. Results： Apoptosis percentages of islet cells and βTc3 cells increased significantly in high concentration glucose cultured medium, mRNA expression of apoptosis-induced gene bax increased significantly and that of apoptosis-resistant gene bcl-2 decreased significantly in the islet cell apoptosis process, and bd-2Poax ratio decreased significantly. The same results were obtained in the study of apoptosis related gene of diabetic rat islet cells by QRT-PCR. Conclusion： High concentration glucose may aggravate low dosage （STZ） diabetes by increasing islet cell apoptosis, and the change of bcl-2/bax mRNA expression ratio may play an important role in this process.

关 键 词：胰岛 细胞 葡萄糖 细胞凋亡 基因表达 

分 类 号：R285.5[医药卫生—中药学] R587.1[医药卫生—中医学]