聚色氨酸膜电极相敏交流伏安法测定肾上腺素  

Determination of epinephrine using phase selective A. C. voltammetry based on poly tryptophan membrane electrode

作　　者：李明华[1] 赵常志[1] 蒙毅[1] 徐福海[1] 

机构地区：[1]青岛科技大学化学与分子工程学院,青岛266042

出　　处：《分析试验室》2006年第4期5-8,共4页Chinese Journal of Analysis Laboratory

摘　　要：研究了聚色氨酸膜电极的制备及其肾上腺素在该修饰电极上的相敏交流伏安特性，建立了相敏交流伏安法测定肾上腺素的电化学分析法。在pH6．0的磷酸盐缓冲溶液中，肾上腺素的相敏交流伏安氧化峰电位在298mV，线性响应范围为1．05×10^-6～3．98×10^-5mol／L，检出限为8．40×10^-7～mol／L。用于药剂中肾上腺素的测定，相对标准偏差小于3．84％，加标回收率为99％～104％，多巴胺、抗坏血酸和尿酸不干扰。A poly （tryptophan） membrane electrode has been prepared and phase selective A.C. vohammetric behavior of epinephrine on this electrode has been investigated. In pH 6.0 phosphate buffer solution, epinephrine has a sensitive A.C. vohammetric response at 298 mV under a condition of 145° phase angle. The linear range for epinephrine is from 1.05 × 10^-6 to 3.98 × 10^-5 mol/L with a correlation coefficient of 0.998 and a detection limit of 8.40 × 10^-7 mol/L. The relative standard deviation （RSD.） is less than 3.8 % （n = 5） and the recovery is in the range of 99% 104% for the determination of medicament, and dopamine, ascorbic acid and urea acid do not interfere the quantitative analysis of epinephrine.

关 键 词：肾上腺素 相敏交流伏安法 聚色氨酸膜电极 

分 类 号：O657.1[理学—分析化学]