液液萃取-气相色谱法测定缓释尿素中吡唑类硝化抑制剂  

Determination of nitrification inhibitor in slow-release urea by liquid-liquicl extraction and gas chromatography

作　　者：左秀锦[1] 夏林[2] 周毅[2] 戴小敏[2] 郑琳[2] 张娜[2] 杨秋玲[2] 马淑慧[2] 陈惠麟[1] 马小军[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116013 [2]大连大学,大连116622

出　　处：《分析试验室》2006年第4期29-32,共4页Chinese Journal of Analysis Laboratory

基　　金：国家"863计划"项目(2001AA246021);中国科学院知识创新重大项目(KZCX1-SW-19)资助

摘　　要：建立了一种快速测定缓释尿素中吡唑类硝化抑制剂（DL-1）的方法-液液萃取．气相色谱法。从5种有机溶剂中选出三氯甲烷作为DL-1的萃取剂，并采用盐析的方法将革取率提高。本文采用内标法，通过SE30柱，氢火焰离子化检测器（FID）定量检测三氯甲烷萃取相中DL-1。萃取率为96．08％～98．10％，平均萃取率为97．33％；回收率为87．96％～90．54％；RSD为0．089％～1．6％，本法测定缓释尿素中的DL-1含量快速、灵敏，便于产品的质量监控。A new method is developed fos the determination of the nitrification inhibitor, based upon liquid-liquid extraction and gas chromatography. Chloroform was found to be the best extractant in the five organic solvents tested, and the efficiency of extraction was improved by salting-out. The method of intemal standard was applied, through a SE 30 column, then a flame-ionization detector （FID） for the GC determination. The extraction efficiency was 96.08% 98.10%. The average extraction efficiency is 97.33% . The recovery was 87.96% - 90.54%, with RSD of 0.089% 1.6%. Then the factory could quickly control their products by this method. The results showed that it was fast, sensitive and convenient to determine the quantity of DL-1.

关 键 词：快速测定 液液萃取 气相色谱 缓释尿素 

分 类 号：O657.7[理学—分析化学]