利用荧光共振能量转移体系测定食品中NO_2^-的研究  

Study of determination of nitrite by fluorescence resonance energy transfer

作　　者：李小燕[1] 李树伟[1] 曾铭[1] 张姝[1] 谢永红[1] 

机构地区：[1]四川师范大学化学与材料科学学院,成都610066

出　　处：《分析试验室》2006年第4期38-41,共4页Chinese Journal of Analysis Laboratory

摘　　要：在λex/λem=450/580 nm,0.1 mol/L的HCl溶液中,番红花红T和吖啶橙能够发生有效的共振能量转移,使得番红花红T荧光增强,同时吖啶橙的荧光猝灭,而NO2-的加入使得两者的荧光强度同时减弱。由此建立了一种新的测定痕量NO2-的方法。结果表明,NO2-在0.02~10μg/mL范围内与染料的荧光强度减弱程度呈良好的线性关系,方法检出限为1.73 ng/mL;该法用于食品中NO2-的测定,回收率为105.0%~112.4%。A new method for the determination of trace nitrite was developed by using energy transfer from acridine orange to safranine. It was found that under the conditions of λcx/λem = 450/580 nm and 0.1 mol/L hydrochloric acid, the effective energy transfer could occur between ST and AO. The fluorescence intensity of AO was reduced and that of ST was strengthen at the same time. But if the nitrite was added to this system, both the fluorescence intensities of AO and ST were reduced. So this system of fluorescence resonance energy transfer was applied to the determination of nitrite. The detection limit of this method was 1.73 ng/mL. The range of determination for nitrite was 0.02 - 10μg/mL and the recoveries were 105 % - 112.4%. The method has been applied to the determination of nitrite in the food with satisfactory results.

关 键 词：吖啶橙 番红花红T 荧光共振能量转移 NO2^- 

分 类 号：O657.3[理学—分析化学]