几个因素对菲律宾蛤仔组织培养的影响  

Several Factors in Tissue Culture for Ruditapes philippinarum

作　　者：李云玲[1] 孙振兴[1] 陈丽媛[1] 李田[1] 徐晓波[1] 张明青 

机构地区：[1]烟台师范学院生命科学学院,山东烟台264025 [2]青岛高利来食品有限公司,山东青岛266555

出　　处：《湖北农业科学》2006年第2期232-235,共4页Hubei Agricultural Sciences

基　　金：山东省自然科学基金资助项目(Y2002D12)

摘　　要：研究了不同消毒剂和培养基对菲律宾蛤仔组织培养的影响。结果表明,在术必泰消毒剂醋酸氯己定(C26H36O4N10Cl2)的有效浓度为100～200μg.mL-1、或复方新洁尔灭中苯扎溴铵(C21H38BrN)的有效浓度为300～600μg.mL-1时,既能有效杀灭粘附在组织块上的细菌,又能保持细胞活性而不影响细胞生长。在D-MEM、M199和RPMI-16403种培养基中,蛤仔的外套膜和鳃细胞均能正常生长,其中D-MEM、M199的培养效果优于RPMI-1640。The influences of different disinfectant and culture medium were studied to the tissue culture of Ruditapes philippinarum. The results showed： When the effective concentration of C26H36O4N10C12 was 100-200μg·mL^-1 on the Shubitai disinfectant, or effective concentration of C21H36BrN was 300-600μg·mL^-1 on the Compound bromo geramine, they could effective kill the bacterium adhered to tissue blocks as well as could keep the activation of cells, not influence made the growth of cells. In the three kind media of the D-MEM, M199 and RPMI-1640, the cells of mantle and gill of clam all could grow normally, effect of D-MEM and M199 were both excelled RPMI-1640.

关 键 词：菲律宾蛤仔 组织培养 消毒剂 培养基 

分 类 号：Q17[生物学—水生生物学] S969.3[生物学—普通生物学]