分化生长因子-8对大鼠移植腹主动脉肌纤维化的作用及其机制  

Myofibrosis mechanism of differentiation growth factor-8 on rat abdominal aorta grafts

作　　者：高成金[1] 熊玮[2] 李幼平[3] 

机构地区：[1]复旦大学附属金山医院烧伤整形科,上海200540 [2]四川大学华西医院泌尿外科 [3]卫生部移植工程与移植免疫重点实验室

出　　处：《中华实验外科杂志》2006年第4期529-531,共3页Chinese Journal of Experimental Surgery

基　　金：国家重点基础研究项目"973"计划资助项目(2003CB515504)

摘　　要：目的探讨分化生长因子(GDF)-8对移植大鼠腹主动脉肌纤维化的抑制作用。方法建立雄性Wistar大鼠至雄性SD大鼠腹主动脉原位移植模型,实验动物随机分为两组:延长冷缺血组(PCI)和对照组,测定术后7、14、21、28 d血管内膜厚度,逆转录-聚合酶链反应(RT-PCR)检测移植血管GDF-8的表达,免疫组织化学(IHC)检测Smad4的表达。结果 PCI组内膜14 d开始增厚,28 d至(381．952±44．334)μm,对照组为(56．898±17．543)μm,差异有统计学意义(P<0．05), 术后PCI组GDF-8 mRNA为对照组的3．6％-33.8％,Smad4蛋白表达高于对照组。结论 GDF-8 在移植动脉肌纤维化过程中发挥重要作用,延长冷缺血时间可下调GDF-8的表达而加速肌纤维化进程。Objective To investigate the role of differentiation growth factor-8 （GDF-8） in inhibiting myofibrosis of abdominal aorta grafts. Methods Male Spague-Dawley （SD） rats received abdominal aorta grafts from male Wistar rats and were randomly divided into two groups： prolonged cold ischemia group （PCI） and control group. Hematoxylin-Eosin （HE） staining was carried out to detect the morphouses of graft aortae and the neointimal thickness was measured. RT-PCR demonstrated the expreasion of GDF-8, Immunohistochimical staining （IHC） was performed to detect the expression of Smad4. Results The intima thickness was increased since 14 days following transplantation in PCI group （P〈0.05）, and reached to （381.952 ± 44.334） μm at 28 days, which was thicker than （56. 898 ± 17. 543） μm in control group （ P 〈 0.05）. The GDF-8 expression in PCI group was only 3.6 % -- 33.8 % of that of control group. There was a much higher expression of Smad4 on the endothelium of PCI group than control group at the same time point. Conclusion Prolonged cold ischemia could accelerate grafts myofibrisis by down-regulating the expression of GDF-8, which plays a key role in the myofibrosis process of rat abdominal aorta grafts.

关 键 词：生长因子-8 腹主动脉 移植 纤维化 

分 类 号：R654.3[医药卫生—外科学]