苯肾上腺素对体外培养人前列腺平滑肌细胞增殖及凋亡的作用  

Effects of phenylephrine on proliferation and apoptosis of in vitro cultured human prostatic smooth muscle cells

作　　者：谢克基[1] 邓向荣[1] 汤平[1] 姜少军[1] 李涛[1] 曾健文[1] 

机构地区：[1]广州医学院附属市一人民医院泌尿外科,510180

出　　处：《中华实验外科杂志》2006年第4期592-593,共2页Chinese Journal of Experimental Surgery

基　　金：广州市教育局资助项目(1999)

摘　　要：目的探讨苯肾上腺素(PE)对体外培养人前列腺平滑肌细胞增殖及凋亡的作用。方法将原代培养得到的3-5代人前列腺平滑肌细胞随机分组,各组加入浓度为0．1μmol／L- 100μmol／L的PE和／或10 μmol／Lα1受体阻滞剂酚妥拉明(Ph),以噻唑蓝(MTT)检测细胞增殖情况,原位凋亡法(TUNEL)检测细胞凋亡情况。结果 PE浓度大于1μmol／L时对前列腺平滑肌细胞有诱导增殖和抗凋亡作用(P均<0．05),并且存在浓度依赖性,r分别为0．90和-0．893(P均< 0．05);10μmol／L Ph能够拮抗PE的诱导增殖和抗凋亡作用(P<0．01)。结论 PE可以通过α1受体信号传导途径诱导体外前列腺平滑肌细胞增殖增加和凋亡减少。Objective To explore the effects of phenylephrine on the proliferation and apoptosis of prostatic smooth muscle cells （SMC）. Methods Human prostatic SMC were primarily cultured and propagated in MCDB-131 medium, then the ceils of the 3rd -5th generation were divided into different groups randomly： control group, phenylephrine groups （sub-divided into 0.1-100 μmol/L groups）, phentolamine （a1-adrenoceptor antagonist） group （10 μmol/L） and combination groups （different concentrations of phenylephrine plus 10 μmol/L of phentolamine）. The effects on cell proliferation and apoptosis were determined by means of MTT cell viability assay and TUNEL assay, respectively. Results The OD values in phenylephrine groups （1-100 μmol/L） were increased dramatically when compared with control group （P 〈 0.05）. The effect of proliferation was in a concentration-dependent manner （r = 0.90, P 〈 0.05） .The apoptotic rate in phenylephrine groups （1-100 μmol/L） was decreased significantly when compared with control group （P 〈 0.05）, also in a concentration-dependent manner （r = -0. 893, P 〈 0. 001）. Conclusion Phenylephrine can induce proliferation and reduce the apoptotic rate of prostatic SMC through the pathway of al-adrenoceptor, and the effects are in a concentration-dependent manner.

关 键 词：前列腺平滑肌细胞 增殖 脱噬作用 苯肾上腺索 

分 类 号：R697.3[医药卫生—泌尿科学]