肾上腺皮质功能对豚鼠实验性哮喘的调控作用  

Recognition ofoxytocin-receptor complex using immobilized antisense peptide

作　　者：魏尔清[1] 唐法娣[1] 陈丽萍[1] 张丽芬[1] 卞如濂[1] 

机构地区：[1]浙江医科大学药理学教研室

出　　处：《中国病理生理杂志》1996年第3期244-248,共5页Chinese Journal of Pathophysiology

摘　　要：抗原（卵白蛋白，ＯＡ）攻击可提高致敏豚鼠血浆皮质醇含量、气道内压和气道内依文思蓝渗出量。甲吡酮（０．１５ｇ／ｋｇ，ｉｐ）降低ＯＡ攻击前后血浆皮质醇含量，并增强ＯＡ引起的气道痉挛和微血管渗漏。ＯＡ攻击后的皮质醇水平与气道内压及依文思蓝渗出间呈显著负相关。氢化可的松可阻断ＯＡ和甲吡酮的作用；甲吡酮对离体气道的平滑肌收缩和微血管渗漏无直接作用。Antisense peptide oxytocin,called AS-OT,whose sequence is encoded in antisense strand of DNA corresponding to the 12 amino-terminal residues of the bovine oxytocin (OT) and its binding protein-neurophysin I(NPI),a region that includes the OT sequence at residues 1～9 were observed.The peptide wasshown to bind selectively to OT and the solubilized complex of OT with receptor protein in plasma membranes from rat mammary gland after delivery.Analyzed with SDS/PAGE method the molecular weight of OT receptor protein was found to be 65kDa.It was suggested that antisense peptide might be used as a tool for affinity chromatography.

关 键 词：氢化考的松 甲吡酮 哮喘 肾上腺皮质 

分 类 号：R562.250.5[医药卫生—呼吸系统]