糖基化LDL与人巨噬细胞作用在动脉粥样硬化发生中的意义  

The role of interaction of gLDL with human macrophage in atherosclerosis

作　　者：冯毅[1] 刘乃丰[1] 陈日新[1] 张丽蓉[1] 

机构地区：[1]南京铁道医学院附属医院心内科

出　　处：《中国病理生理杂志》1996年第4期360-363,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金

摘　　要：本文观察了体外糖基化ＬＤＬ在人巨噬细胞（Ｍφ）中的代谢和细胞内脂质含量变化。Ｍφ的ＬＤＬ受体最大高亲和结合（Ｂ＿（ｍａｘ））为５５５．９１ｍｇ／ｇ细胞蛋白，亲和常数（Ｋｍ）为５９．４８ｍｇ／Ｌ。糖基化ＬＤＬＢ＿（ｍａｘ），为４３４．７８ｍｇ／ｇ细胞蛋白，Ｋｍ为１７．８２ｍｇ／Ｌ．Ｍφ结合，摄入和降解糖基化ＬＤＬ明显强于正常ＬＤＬ组。与糖基化ＬＤＬ孵育的Ｍφ胞内总胆固醇含量显著高于对照组（Ｐ＜０．０５）。结果提示人巨噬细胞代谢糖基化ＬＤＬ与正常ＬＤＬ途径不同，此过程伴细胞内脂质积聚易于形成泡沫细胞。The in uitro metabolic proeess of gLDL in human macrophage and thechange of total cholesterol contained in the eell was observed.The B(max) of LDL reeeptoron the eell was 555.9 1 mg/g cell protein,the Km was 59.48mg/L.The B(max) of glycosylatedLDL was 434.78 mg/g eell protein,the Km was 17.82mg/L. The binding,internalizationand degradation of gLDL by the eell was higher than that of native LDL,The totalcho lesterol in the eell incubating with gLDL was higher than that with native LDL(P<0.05).The result indicated that the metabolic proeess of gLDL in hulnan macrophagewas different from that of the native LDL。This process accompanied the deposition oflip id which n1ade the eell beco me foam cell much easier.

关 键 词：低密度脂蛋白 动脉粥样硬化 巨噬细胞 

分 类 号：R543.502[医药卫生—心血管疾病]