肠缺血性休克犬血清PLA_2活性和MDA含量变化及促甲状腺素释放激素对其影响  

Changes of serum PLA2 and serum MDA in dogs intestinal ischemia shock and effects of TRH on them

作　　者：胡志前[1] 王元和[1] 吴德敬[1] 徐学俊[1] 吴先仁[1] 宫钦志[1] 

机构地区：[1]上海第二军医大学长征医院普外科

出　　处：《中国病理生理杂志》1996年第4期392-395,共4页Chinese Journal of Pathophysiology

摘　　要：本实验采用同时测定血清磷脂酶Ａ２（ＰＬＡ２）和脂质过氧化代谢终产物丙二醛（ＭＤＡ）的方法，动态观察两者在犬肠缺血性休克中的作用及促甲状腺素释放激素（ＴＲＨ）对它们的影响。结果显示：休克后，血清ＰＬＡ＿２活性和血清ＭＤＡ含量均明显升高，与平均动脉压呈显著负相关。而两者间则存在显著正相关关系，两者协同参与了肠缺血性休克的发生发展过程。静脉注射ＴＲＨ能有效地抑制血清ＰＬＡ。活性升高和明显地降低血清ＭＤＡ含量，这可能是ＴＲＨ抗休克机理的一个重要因素。By testing serum phospholipase A2 (PLA2)activity and serum malondialdehyde(MDA)coneentration simuItaneously、the role of them in dogs intestinal ischemia shockand the effects of thyrotropin-releasing hormone(TRH)on them were studied The model ofshock was created by occluding both Superior mesenteric artery and vein(SMAv)for a 240min period and releasing SMA.V. The TRH-treated dogs(n=5)were given TRH(total 5mg/kg)iv at 30min before releasing SMA,V,and the control dogs(n=5)received salineequivalent.The resuIts showed that serum PLA2 cativity and serum MDA concentrationwere increased significantly after shock and were correlated with decrease of mean artery pressure(P<0.01).While there was a definite positive correlation between serum MDA coneentrationand serum PLA2 activity,which suggested that both PLA2 and LPO are involved in thepathophysiological changes of intestinal ischemia shock coordinately.Serum PLA2 activity ofthe TRH-treated dogs increased also,but was lower than that ofthe control dogs after treatment(P<0.01 or <0.001).Serum MDA of the TRH-treated dogs did not increase and was lowerthan that of the control dogs after treatment(P<0.05 or < 0.00 1).These results suggestedthat one important factor of the antishock mechanisnis of TRH in canine intestinal ischemiashock might be its effective suppression of serum PLA2 activity and alleviation oflipoperoxidation danlage.

关 键 词：丙二醛 磷酶类 肠缺血性休克 TRH 

分 类 号：R605.971[医药卫生—急诊医学]