白细胞介素-2前体脂质体的初步研究  

Preliminary evaluation of interleukin-2 pro-liposome

作　　者：韩庆惠 李连会[1] 

机构地区：[1]上海新药研究开发中心,上海201203

出　　处：《中国新药杂志》2006年第5期354-356,共3页Chinese Journal of New Drugs

基　　金：国家科技攻关计划项目(2004DFBA0008)

摘　　要：目的:制备白细胞介素-2(IL-2)前体脂质体并考察其稳定性。方法:采用冷冻真空干燥法制备IL-2前体脂质体,采用Sephadex^(TM) G-50柱分离脂质体,MTT比色法测定活性,激光粒度仪测定粒径。结果:制备的IL-2前体脂质体为白色的非定型粉末,粒径分布在0．1～1．0μm,包封率为95．5%,活性5．0×10~6IU·g^(-1)。结论:本方法制备的前体脂质体制剂有较高的包封率,贮存稳定,粒度分布良好。Objective ：To prepare and characterize the interleukin-2 pro-liposome. Methods ：The IL-2 pro-liposome was prepared by the vacuum freeze-drying system and then purified by the Sephadex^TM G-50 column. The interleukin-2 activity was measured using the MTT colorimeter, and the particle diameter was determined by laser diffraction systems （ HORIBA LA-920）. Results ：The IL-2 pro-liposome was non-finite white powder with the particle diameter of 0.10 - 1μm and the activity of five millions unit/g. The inclusion rate of IL-2 pro-liposome was 95%. Conclusion：The Interleukin-2 pro-liposome with higher inclusion and stability was achieved.

关 键 词：前体脂质体 包封率 冷冻真空干燥系统 

分 类 号：R944.9[医药卫生—药剂学] R392.12[医药卫生—药学]