薄层刮板-荧光分析法在中药复方制剂质量控制中的应用  

Application of Thin Layer Chromatograph-Fluorescent Method in Quality Control of Herbal Preparations

作　　者：李玲[1] 刘明珠[1] 徐卫明 

机构地区：[1]第二军医大学药学院,上海200433

出　　处：《中草药》1996年第5期275-277,共3页Chinese Traditional and Herbal Drugs

摘　　要：用薄层刮板－荧光分析法检测中药复方制剂之君药丁公藤ErycibeobtusifoliaBenth的有效成分东莨菪亭（scopoletin）的含量，克服了薄层色谱干扰，取得了准确、可靠的结果。本文的研究为中药复方制剂的质量控制提供了一种较好的分析手段。A thin layer chromatograph-fluorescent method was used to assay content of scopoletin in Erycibe obtusi folia Benth.. The method showed that it not only can minimize analytical errors in thin layer scan and column contamination in HPLC,but also eliminated mutual interference of components in the preparation. It provides a good assay technique for quality control of hebal preparations. The method was carried out on thin layer plates of silica gel H(40μ)with benzene-ether (1. 1: 1 )as the developer,at an excitation spectrum of 347nm,fluorescence spectrum of 420nm and a temperture of 25°±1℃.The method showed good linearity(r= 0. 9998, n = 5)in the range of 0.08~0. 40μg/μl of scopoletin and the average recovery was 98. 52 % ~ 102. 67 % with RSD< 1. 5% (n = 5).

关 键 词：丁公藤 东莨菪亭 薄层刮板 荧光分析法 

分 类 号：R286.1[医药卫生—中药学]