丙型肝炎病毒感染者肝组织中病毒相关抗原的表达  

The expression of hepatitis C virus-related antigens in the liver tissues of patients infected by HCV

作　　者：赵西平[1] 杨东亮[1] 程玉芳 彭志海[1] 李淋[1] 李方和[1] 唐振亚[1] 郝连杰[1] 

机构地区：[1]武汉同济医科大学附属同济医院

出　　处：《中华传染病杂志》1996年第2期81-84,共4页Chinese Journal of Infectious Diseases

摘　　要：为研究丙型肝炎病毒（ＨＣＶ）结构和非结构抗原在肝组织中的分布状况及其相互关系，应用免疫组织化学方法，以多克隆抗－ＨＣＶ和单克隆抗－ＨＣＶ－ＮＳ３，ＮＳ５及ＣＰ１０，对一组ＨＣＶ感染者石蜡包埋肝组织中ＨＣＶ抗原进行检测。结果发现：应用四种抗体自ＨＣＶ感染者肝组织中均检出相应抗原，检出率分别为３２．２％，２４．７％，５９．１％和５２．７％；ＨＣＶ－ＮＳ５和ＣＰ１０表达较强，而ＨＣＶ－ＮＳ３阳性程度较弱；阳性颗粒定位于肝细胞胞浆；阳性细胞分布呈散在，弥漫状和簇状三种形式；ＨＣＶ－ＮＳ３和ＣＰ１０的表达与炎症反应无明显解剖学关系，但ＨＣＶ－ＮＳ５阳性细胞较常见位于炎症灶旁或灶中。结果提示：ＨＣＶ各成份在肝组织中的表达水平存在差别；ＨＣＶ－ＮＳ５的表达可能与ＨＣＶ致病机制有关。To understand the distribution of structional and non-structional components of hep-atitis C virus(HCV)in liver tissue,we detected HCV antigens by immunohistochemical method us-ing polyclonal antibody to HCV and monoclonal antibodies to HCV-NS3,NS5 and cOre(CP10)re-spectively.The results showed that HCV antigens in liver tissues could be detected by using all thefour antibodies.The positive rates were 32.2%,24.7%,59.1%and 52.7%respectively,the stainsignals of HCV-NS5 and CP10 were stronger than that of HCV-NS3,and the positive granules werelocated in the cytoplasm of liver cells.Three types of distribution of positive cells were found:scat-tering,clustering and diffusive. No obvious topographical relationship was observed between the ex-pression of HCV-NS3 or CP10 and inflammatory reaction,but necrosis of liver cells or/and inflam-matory infiltration could be found around the HCV-NS5 positive cells. These results indicated thatthe expression of HCV antigens in liver cells was not equal and the expression of HCV-NS5 mighttake part in the pathogenesis of HCV.

关 键 词：丙型肝炎病毒 免疫组化 抗原 

分 类 号：R373.2[医药卫生—病原生物学]