铁缺乏大鼠畸变产物耳声发射与耳蜗毛细胞的改变  

Changes of distortion product otoacoustic emissions and cochlear hair cells in jron-defident rats

作　　者：田树昌[1] 孙爱华[1] 邱杰[1] 王秋莎 范静平[1] 林顺涨 胡建道[3] 沈勤峰[4] 

机构地区：[1]上海长征医院耳鼻咽喉科,上海200003 [2]常州第二人民医院高压氧科 [3]解放军第四一三医院耳鼻咽喉科 [4]吴江市第二人民医院耳鼻咽喉科

出　　处：《中华航海医学与高气压医学杂志》2006年第1期6-9,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：国家自然基金(30472124)

摘　　要：目的观察不同饲养期铁缺乏大鼠畸变产物耳声发射(DPOAE)的改变与耳蜗毛细胞改变的关系,探讨铁缺乏引起听毛细胞损伤的可能机制。方法以缺铁饮食饲养法复制缺铁Wistar大鼠模型33只,标准饮食喂养33只作对照。每组分别于喂养第4,8,12周各随机取11只测双耳DPOAE幅值,并处死做耳蜗扫描电镜。结果缺铁组第4周,36．4％耳数(8／22)出现DPOAE幅值中、高频率段下移,但扫描电镜下未发现毛细胞病理改变;缺铁至第8周,59．1％耳数(13／22)出现DPOAE幅值中、高频率段程度较重的下移,13耳中,3个耳蜗出现不同程度外毛细胞静纤毛散乱、扭曲、倒伏、融合等病理改变;缺铁至第12周,55．6％耳数(10／18)DPOAE幅值中、高频率段下移。10耳中,5个耳蜗出现不同程度外毛细胞静纤毛散乱、扭曲、倒伏、融合,缺失等病理改变,病变范围扩大。结论铁缺乏可使Wistar 大鼠DPOAE以及耳蜗毛细胞发生改变,DPOAE改变发生在毛细胞改变之前,DPOAE幅值下降到一定程度延缓或停止,但毛细胞病理改变仍有加重趋势。Objective To obsreve DPOAE change in rats at different iron-deficient stages. Methods Sixty-six healthy Wistar rats were randomly divided into control group （ 33 rats） and iron-deficient group （ 33 rats）. The iron-deficient group were fed with iron-different diet. DPOAEs were measured and cochlear hair cell were examined by scanning electron microscopy at the 4th ,8th and 12th week in both groups. The iron-deficient diet was continued to the 8th week but stopped at the 12th week. Results A significant decrease of DPOAE threshold levers at 2 kHz,4 kHz in iron-deficient group was obserred at the 4th week, More frequencies were affected as the iron-deficient diet was continued to the 8th week, but stop at the 12th week. Conclusions Irondeficient can effect the function and structure of cochlear hair cells.

关 键 词：铁缺乏 WISTAR鼠 畸变产物耳声发射 毛细胞 

分 类 号：R764[医药卫生—耳鼻咽喉科]