HPLC测定治糜灵泡腾片中盐酸小檗碱的含量  

Determination of Berberine Hydrochloride in Zhimiling Effervescent Tablets by HPLC

作　　者：仇雅静[1] 

机构地区：[1]江苏省泰州药品检验所,江苏泰州225300

出　　处：《现代中药研究与实践》2006年第1期47-48,共2页Research and Practice on Chinese Medicines

摘　　要：目的建立治糜灵泡腾片中盐酸小檗碱的含量测定方法。方法高效液相色谱法。采用Symme-try C18柱(3.9 mm×150 mm,5μm),流动相为乙腈-0.05 mol/L磷酸二氢钠溶液(用磷酸调节pH值至3)(30∶70),检测波长为270 nm。结果此方法线性关系良好,平均回收率为99.3%。结论此方法简便、准确,可用于测定治糜灵泡腾片中盐酸小檗碱的含量。Objective To establish a HPLC method for the determination of Berberine hydrochloride in Zhimiling effervescent tablets. Methods A Symmetry C18 column （3. 9 mm×150 mm,5 μm） was used with the mobile phase of acetonitrile-0.05 mol · L^-1 sodium dihydrogen phosphate solution（adjust pH to 3 with phosphonic acid）（30： 70）. The detective wavelength was at 270nm. Results The linearity of thismethod was well with the average recovery of 99.3%. Conclusion The method is simple and accurate and can be used for the determination of the contents of Berberine hydrochloride in Zhimiling effervescent tablets.

关 键 词：治糜灵泡腾片 盐酸小檗碱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]