淋巴丝虫感染中T淋巴细胞的免疫应答──白细胞介素2和γ干扰素的体外诱生分析  

T CELL IMMUNE RESPONSE IN LYMPHATIC FILARIASIS ANALYSIS OF PARASITE ANTIGEN INDUCED IL 2 AND IFN γ PRODUCTION

作　　者：陈志琳[1,2,3,4] 沈一平[1,2,3,4] 陈淑贞 张耀娟[1,2,3,4] 管晓虹 尤丽芬[1,2,3,4] 杨维平 王增贤[1,2,3,4] 

机构地区：[1]扬州大学医学院寄生虫学教研室 [2]南京医科大学寄生虫学教研室 [3]南京医科大学微生物学教研室 [4]安徽医科大学寄生虫学教研室

出　　处：《中国寄生虫学与寄生虫病杂志》1996年第1期50-53,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金

摘　　要：目的：探讨淋巴丝虫感染中Ｔ淋巴细胞的免疫应答机制。方法：检测安徽省班氏丝虫病流行区人群及感染马来丝虫的长爪沙鼠体外诱生的白细胞介素２（ＩＬ－２）和γ干扰素（ＩＦＮ－γ）。结果：对非特异性有丝分裂原ＰＨＡ及ＣｏｎＡ的刺激，各组人群外周血单核细胞（ＰＢＭＣ）产生ＩＬ－２及ＩＦＮ－γ水平无显著差异。对马来丝虫成虫抗原的刺激，微丝蚴血症者ＰＢＭＣ产生ＩＬ－２及ＩＦＮ－γ水平比流行区无症状、无微丝蚴血症者显著低下，长爪沙鼠实验结果与人群的相似。结论：本实验提示，班氏丝虫病流行区微丝蚴血症者及马来丝虫微丝蚴血症长爪沙鼠的Ｔ细胞对丝虫抗原处于一种低应答状态，且这种受抑制的Ｔ细胞主要是Ｔｈ１亚群细胞。AIM: To investigate the mechanism of T cell immune response in lymphatic filariasis. METHOD: IL 2 and IFN γ production in peripheral blood mononuclear cells (PBMC) were examined in vitro from 31 patients with microfilaremia, 32 amicrofilaremic individuals living in bancroftian filariasis endemic area of Anhui Province and 32 controls living in non endemic area. The jirds infected with B.malayi were also examined for IL 2 and IFN γ production in spleen cells. RESULTS: In each group of human subjects, the production of IL 2 or IFN γ in response to PHA and ConA did not differ significantly. When B.malayi adult antigen (BmA) induced IL 2 or IFN γ production was examined, the amicrofilaremic individuals generated significantly more cytokines than did the patients with microfilaremia. In jirds, the results were similar to those in humans. CONCLUSION: These results suggest that there exists T cell hyporesponsiveness to BmA in patients with microfilaremia from bancroftian filariasis endemic area and in jirds with microfilaremia of B.malayi.

关 键 词：丝虫病 白细胞介素2 Γ干扰素 T细胞 免疫应签 

分 类 号：R532.150.3[医药卫生—内科学]