离体肾小管低氧、氧复苏损伤中的脂类介质和磷脂酶A_2  

Variation of lipids and phospholipase A_2 activity during hypoxic and reoxygenation of tubular cells in vitro

作　　者：姜傥[1] 范瑾瑾[1] 关伟明[1] 谭炳德 董秀清[1] 

机构地区：[1]中山医科大学肾脏病研究所,中山医科大学检验科,广东省职业病防治院

出　　处：《中华肾脏病杂志》1996年第1期20-24,共5页Chinese Journal of Nephrology

基　　金：国家自然科学基金

摘　　要：从体外制备肾小管上皮细胞低氧、氧复苏模型入手，着重分析了低氧、氧复苏损伤过程中磷酯酶（ＰＬ）Ａ＿２的活性变化，ＰＬＡ＿２活性变化与细胞内钙稳态、脂质过氧化产物（ＬＰＯ）及ＰＧｓ、ＴｘＡ等脂类介质之间的关系，以探究低氧、氧复苏致肾小管上皮细胞损伤的本质。结果发现，在低氧及低氧、氧复苏时，ＰＬＡ＿２活性逐渐升高，尤其在氧复苏过程中，ＰＬＡ２活性升高明显；与此同时，［Ｃａ］ｉ自始至终处于高水平状态，两者呈明显相关关系（ｒ＝０．６８，Ｐ＜０．０１）。人为调节细胞外钙离子浓度也发现，Ｃａ浓度可明显影响ＰＬＡ＿２活性。ＰＬＡ＿２的激活呈明显Ｃａ依赖性。推测在低氧、氧复苏性损伤中，ＰＬＡ＿２的激活极有可能是由于低氧、氧复苏造成的细胞内钙离子浓度增高所致。ＰＬＡ＿２的损伤作用可能与由其引起的脂质过氧化及脂类介质的产生与释放有关。ＣｙＡ可明显阻断ＰＬＡ＿２的激活以及过氧化脂质、脂类介质的大量产生和堆积，缓解低氧、氧复苏所造成的细胞损伤。The study took renal tubular cells,incubated in hypoxic and reoxygenation enviroment,as a mod-el. Activity of phospholipase A2 in cell injury of hypoxic and reoxygenation,the relationship between PLA2 activityand cytosolic calcium hometasis,and、the level of lipid peroxidation products,and the level of PGs and TxA wereanalysed.The results showed that PLA2 activity and cytosolic calcium concentration increased gradually during hy-poxic and reoxygenation,expecially in reoxygenation processes.Different exogenous Ca concentration treatmentof hypoxic proximal tubules could remarkably affect its PLA2 activity.The activation of PLA2 was companied byincreasing level of LPO,PGI,TxA. When tubular cells model treated with cycrosporine A,a kind of PLA2 in-hibitors,PLA2 activity was inhibited and the content of LPO and lipid mediums was significantly decreased.

关 键 词：低氧 氧复苏 肾小管细胞 磷脂酶A2 肾功能衰竭 

分 类 号：R692.502[医药卫生—泌尿科学]