N^+离子注入对同源四倍体水稻诱导效果的影响  

Effect of N^+ Implantation on Inducing Autotetraploid Rice

作　　者：李玉峰[1] 黄群策[1] 

机构地区：[1]湖南科技大学生命科学院,湖南湘潭411201

出　　处：《杂交水稻》2006年第2期69-71,共3页Hybrid Rice

基　　金：国家863资助项目(SZ-01-01-03)

摘　　要：以IR36、紫血稻、多胚苗品系APⅣ为材料,研究了N+注入对秋水仙素诱导水稻同源四倍体效果的影响。结果表明,当秋水仙素处理24 h时,经剂量为0.52×1016N+/cm2的N+注入处理,种芽成活率提高,但在其后代中没有筛选到四倍体水稻植株,而用剂量为6.34×1016N+/cm2的N+注入处理的后代中发现了四倍体水稻植株;当秋水仙素处理48 h或48 h以上时,经剂量为0.52×1016N+/cm2的N+处理不但使种芽的成活率提高,而且还提高了诱导四倍体的效率,而剂量为6.34×1016N+/cm2的N+注入处理会使四倍体诱导效率明显下降。The effect of N^＋ ion implantation on inducing autotetraploid rice by colchicum liquor was studied with IR36, Zixuedao and poly-embryo rice APⅣ as the plant materials. The results showed that when the seeds was treated by 24 h of colchicum liquor, the treatment of 0.52 ×10^16N^＋/cm^2 increased the survival rate of seed buds, but no autotetraploid rice plants were found among the treated population, while the treatment of 6.34 ×10^16N^＋/cm^2 induced 2 and 1 tetraploid rice plants in IR36 and Zixuedao, respectively. When the treating time of colchicum liquor was 48 h or more, the treatment of 0.52 ×10^16N^＋/cm^2 increased not only the survival rate of seed buds but also the inducing efficiency of tetraploid rice, while the treatment of 6.34 ×10^16N^＋/cm^2 greatly decreased the inducing efficiency of tetraploid rice.

关 键 词：氮离子注入 秋水仙素 同源四倍体水稻 诱导效果 

分 类 号：Q689[生物学—生物物理学] S121[农业科学—农业基础科学]