高效液相色谱法测定复方黄酮胶囊中槲皮素的含量  

Determination of quercetin in compound flavone capsules by HPLC

作　　者：陈英[1] 危华玲[1] 范吕林[1] 

机构地区：[1]广西壮族自治区人民医院,广西南宁530021

出　　处：《中国医院药学杂志》2006年第4期386-387,共2页Chinese Journal of Hospital Pharmacy

基　　金：广西壮族自治区卫生厅课题(编号:Z2003081)

摘　　要：目的：对复方黄酮胶囊中的有效成分槲皮素进行含量测定。方法：高效液相色谱（HPLC）法，C18柱，以甲醇-0．4％磷酸（45：55）为流动相，流速为0．9mL·min^-1，检测波长为360nm。结果；槲皮素在0．3248～3．2480μg范围内与峰面积线性关系良好，r=0．9998，平均回收率为99．13％，RSD为0．78％（n=6）。结论：本方法简便，准确，重现性好。OBJECTIVE To establish a method for determining the content of quercetin in compound flavone capsules. METHODS The suntek 18 column with methanol-0. 4% phosphorous acid （45：55） as the mobile phase was used. The flow rate was 0. 9 mL·min^-1 and the detective wavelength was 360 nm. RESULTS The linear range of calibration curve was 0. 324 8 - 3. 248 0μg,for the quercetin （r= 0. 999 8）. The average recovery was 99. 13% and the RSD was 0. 78% （n = 6）. CONCLUSION The method is simple,and accurate with a good reproducibility.

关 键 词：复方黄酮胶囊 槲皮素 高效液相色谱法 含量测定 

分 类 号：R965[医药卫生—药理学]