高效液相色谱法测定参茸口服液中人参皂苷Rg_1含量  

Determination of ginfenoside Rg_1 content in Renax oral liquids with HPLC

作　　者：刘丰丰[1] 青磊[2] 张倩[1] 王海宁 

机构地区：[1]贵阳医学院附属医院,贵州贵阳550004 [2]中国人民解放军第44医院,贵州贵阳550009 [3]贵州省药品检验所,贵州贵阳550004

出　　处：《中国医院药学杂志》2006年第4期433-435,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的：测定参茸口服液人参皂苷Rg1的含量。方法：用高效液相色谱法测定Rg1的含量。色谱分析采用Diamonsil^TM（钻石），C18色谱分析柱，流动相为乙腈磷酸二氢钾缓冲液（25：75）pH3．5，柱温30℃；紫外检测波长为203nm流速为1．0mL·min^-1。实验数据用Gilson 715液相色谱工作站处理。以峰面积外标法定量。结果：人参皂苷Rg1进样量在0．10042～0．90378μg，与峰面积分值呈良好的线性关系。r=0．9996（n=5），平均回收率为100．06％。结论：此方法简便、快速、精度高、分离度好。可作为参茸口服液成品中人参皂苷Rg1的定量分析。OBJECTIVE To determine ginsenoside Rg1 in Renax oral liquids. METHODS Rg1 was tested by HPLC using C18 chromfographic column with aeetonifrile-0. 05% phosphoric acid（25：75） as mobile phase. The column temperature was 30℃ ,the wavelength for detection was 203 nm, flow rate was 1.0 mL·min^-1. The experiment data were treated with Gilson 715 chromatographic workstation and calculated by peak area and external standard method. RESULTS The recovery of schizandrin was 100.06%,RSD was 1.14%. CONCLUSION The method is found to be convenient,sensitive and accurate.

关 键 词：人参皂苷RG1 高效液相色谱法 参茸口服液 

分 类 号：R927.2[医药卫生—药学]