针刺对自由运行状态金黄地鼠视交叉上核内5-HT_7受体功能的影响  

Regulating Effects on Electro-acupuncture on 5-HT Receptors in Suprachiasmatic Nucleus of Free-running Hamsters

作　　者：蔡定均[1] 宋开源[1] 刘旭光[1] 周奇志[1] 赵纪岚[1] 魏焦禄[1] 薛红[1] 

机构地区：[1]成都中医药大学针灸推拿学院,四川成都610075

出　　处：《成都中医药大学学报》2006年第1期34-36,共3页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助(编号:30171180)

摘　　要：目的:探讨针刺导引节律的5-HT受体转导机制。方法:金黄地鼠经驯化进入自由运行状态后,于DD第三天CT9给予电针、8-OH-DPAT、光脉冲导引、捆绑导引刺激。刺激后2 h取样,应用放免法测定视交叉上核(SCN)内5-HT7受体功能的变化。结果:电针和8-OH-DPAT组KD值显著低于捆绑对照组;光脉冲导引组的KD值明显高于捆绑对照组,差异有统计学意义;说明电针和8-OH-DPAT均能使5-HT7受体的亲和力增强,而光脉冲刺激则可抑制其亲和力。结论:针刺通过提高5-HT7受体的活性,实现在主观白天的相位超前性调整。Objective： To research the regulating effects of electro- acupuncture on 5 - HT receptors in the suprachiasmatic nucleus （SCN）. Methods： After free- running, hamsters were divided into electro- acupuncture group, 8 - OH - DPAT group, photic stimuli group and binding group rando mLy. Electro - acupuncturing ＂Baihui＂ （Du20）, ＂Changqiang＂ （Dul）, system injecting 8 - OH - DPAT, photic and binding stimuli were given respectively at circadian time 9 （cr9） in the third day after constant darkness. The regulating effects of electro - acupuncture on 5 - HT7 receptors in SCN were detected by using radioligand binding assay of receptors. Results： The dissociation constant （KD） of electro - acupuncture and 8 - OH - DPAT groups was significantly lower than that of binding group. And the KD of photic stimuli group was remarkably higher than that of binding group. It means that electro - acupuncture and system injecting 8 - OH - DPAT improved affinity of 5 - HT7 receptors but photic stimuli decreased it in. Conclusion： electroacupuncture induced phase advance at subjective day in hamster spontaneous behavior by activating 5 - HT7 receptors in SCN.

关 键 词：针刺 5-HT7受体 SCN 近似昼夜节律 

分 类 号：R245.9[医药卫生—针灸推拿学] R338.63[医药卫生—中医临床基础]