妍婷颗粒对输卵管炎性不孕大鼠模型ICAM-1的影响  

Influence of Yanting Granules on ICAM-1 in rat model of salpingitic infecundity

作　　者：杨新鸣[1] 侯丽辉[2] 吴效科[2] 

机构地区：[1]黑龙江中医药大学,黑龙江150040 [2]黑龙江中医药大学附属第一医院妇产科

出　　处：《北京中医药大学学报（中医临床版）》2006年第2期1-4,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：黑龙江省科学技术厅攻关课题(No.GC01C142-02)

摘　　要：目的探讨中药妍婷颗粒对输卵管炎性不孕大鼠细胞间黏附分子-1(intercellular adhesion molecule,ICAM-1)的影响及其疗效与机理。方法用混合菌接种法制作输卵管炎性不孕大鼠模型,以妍婷颗粒进行治疗,分为灌肠和灌胃两组,治疗后用ELISA法检测血清中可溶性细胞间黏附分子-1(solubility intercellular adhesion molecule,sICAM-1)水平和用免疫组织化学方法检测大鼠ICAM-1蛋白表达。结果大鼠ICAM-1蛋白表达和血清中sICAM-1水平灌胃组与灌肠组均较模型组降低(P<0.05或P<0.01),且灌肠组较灌胃组亦低(P<0.05)。结论妍婷颗粒对大鼠ICAM-1蛋白表达和血清中sICAM-1水平有调节作用,从而对大鼠炎性不孕具有很好的治疗作用。Objective To investigate the influence, curative effect and mechanism of Yanting Granules on intercellular adhesion molecule-1 （ICAM-1） in rat model of chronic salpingitic infecundity. Methods The rat model of chronic salpingitic infecundity was established by using mixed bacteria inoculation and then treated with Yanting Granules. The rats were divided into the coloclysis goup and oral group. After the treatment the serum level of solubility intercellular adhesion molecule-1 （sICAM-1） was detected with ELISA method and the ICAM-1 expression in rats was detected with immunohistochemistry. Result The serum level of sICAM-1 and ICAM-1 expression in rats in the coloclysis group and oral group decreased compared with that of the model group （P〈0.05 or P〈 0.01）. Those were lower in the coloclysis group than in the oral group （P〈0.05）. Conclusion Yanting Granules has an integrated ef- fect on the serum level of sICAM-I and ICAM-lexpression in rats and therapeutic effect on rat salpingitic infecundity.

关 键 词：输卵管炎性不孕 妍婷颗粒 细胞间黏附分子-1 蛋白表达 可溶性细胞间黏附分子-1 

分 类 号：R271.14[医药卫生—中医妇科学]