温和气单胞菌单克隆抗体的制备及特性分析  

Preparation and Characterization of Monoclonal Antibodies to Aeromonas Sobria

作　　者：黄钧[1] 梁明龙[1] 朱芸[1] 

机构地区：[1]广西大学动物科技学院,广西南宁530004

出　　处：《淮阴师范学院学报（自然科学版）》2006年第1期74-77,共4页Journal of Huaiyin Teachers College;Natural Science Edition

摘　　要：利用细胞融合技术建立了5株分泌抗温和气单胞菌CR79-1-1株单克隆抗体(McAb)的杂交瘤细胞系,通过特异性鉴定获得两株与参考菌株中的温和气单胞菌发生反应,但与嗜水气单胞菌以及鳗弧菌、爱德华氏菌、克鲁氏耶尔森菌、大肠杆菌、荧光假单胞菌均不反应的杂交瘤细胞系,分别命名为2G3和1A4.快速酶联免疫分析法(ELISA)测得2G3和1A4的亲和常数分别为1.72×108和5×108M-1;应用方阵配对实验证实,2株单抗针对特异性抗原上不同表位.Five hybridoma cell lines （ 1A4, 2C7, 3G9,3D5 and 2G3） preducing monoclonal antibodies （McAbs） against Aeromonas sobria CR79-1-1 strain were established by the fusion technique. The specificity analysis by indirect ELISA showed that 2G3 and 1 A4 lines can react with A. sobria. None of them had a cross reaction with A. hydrophila, Vibrio anguillarum, Edwardsiella, Yersinia ruckeri, Escherichia coli and Pseudomonas fluolescens. Affinity constants of 2G3 and 1 A4 are 1. 72 × 10^8 and 5 × 10^8 M I, respectively. Different epitopes of A.sobria were recognized by the two McAbs.

关 键 词：温和气单胞菌 单克隆抗体 表位 酶联免疫吸附分析 

分 类 号：S948[农业科学—水产养殖]