两种CPG血小板聚集试验在血小板捐献者筛查中的应用  

Evaluation of the Platelet Function of Platelet Donors by Using Two Thrombocyte Aggregation Test with Cationic Propyl Gallate(CPG) as the Inducer

作　　者：史广耀[1] 黄成垠[1] 蔡莉[1] 肖健宇[1] 唐荣才[1] 盛大祥[1] 

机构地区：[1]江苏省血液中心,南京210042

出　　处：《临床输血与检验》2006年第2期97-99,共3页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的探讨以阳离子没食子酸丙酯(CPG)为血小板激活剂的血小板聚集实验在单采血小板献血者血小板功能筛查应用中的可行性,并对血小板聚集仪法和薄片法(SPAT)进行比较。方法(1)以CPG为血小板激活剂,分别用血小板聚集仪法和薄片法测定30名自愿者服用阿司匹林前和服药24h后的血小板聚集功能,以确定服药与否的临界值;(2)应用血小板聚集仪法和薄片法同时检测483例单采血小板捐献者的血小板聚集功能,调查血小板献血者血小板功能缺陷的发生率。结果(1)服用阿司匹林的自愿者服药24h后,CPG诱导的血小板聚集率(聚集仪法)均显著降低,尤以180s时的血小板聚集率减低最为显著,由服药前的45.4%±9.5%降低为10.1%±5.0%(P<0.001)。23例自愿者服药24h后血小板聚集时间(薄片法)显著延长,由服药前的(64.5±13.9)s延长为(178.8±16.3)s(P<0.001)。血小板功能缺陷的临界值(cutoff)确定为:血小板聚集仪法为测定时间180s时的血小板聚集率小于20%;薄片法为血小板聚集时间≥150s;(2)483例血小板捐献者中,血小板聚集仪法检出血小板聚集功能不良者25例(5.18%);薄片法为5例(1.03%)。结论以CPG为激活剂的血小板聚集实验能有效检出阿司匹林服药者或其他因素导致的血小板聚集功能不良者,适用于单采血小板捐献者血小板功能筛查,以血小板聚集仪法优于薄片法。Objective To investigate the feasibility of the application of cationic propyl gallate（CPG） as inducer of platelet aggregation for scanning the platelet function of single-donor plateletpheresis and compare the efficiencies of aggregometer and the slide platelet aggregation test （SPAT）. Methods （1）LG-PABER platelet aggregometer and SPAT were used to examine in platelet aggregation before and 24 h after administration of aeetylsalicylic acid（ASA） after induction by CPG for determining the cut off value to discriminate the intake of ASA; （2）483 healthy platelet donors＇ plateht aggregation were measured to investigat the incidence of defective platelet function among donors. Results （1）The platelet aggregation rate was significantly decreased in the LG-PABER aggregometer test 24 h after the administration of ASA,which decreased from 45.4%±9.5% to 10. 1%±5.0%（P〈0. 001） at 180 s induced by CPG. The platelet aggregation time of 23 volunteers after 24 h administered ASA was significantly prolonged with slide platelet aggregation test after induction by CPG, which ascended from （64.5± 13.9） s to （178.8±16.3） s（P〈0. 001） ; （2）The cut off point of platelet aggregation of the LG-PABER aggregometer test and the slide platelet aggregation test were fixed on the plateht aggregation 〈20% at 180 s and 〉/150 s respectively, the donors who take ASA can be selected effectively; （3） Among 483 donors,25 （5.18%） defective platelet aggregation function were detected by LG-PABER aggregometer test and 5 （1.03%） easesplatelet dysfunction donors were detected by the slide platelet aggregation test. Conclusion Platelet aggregation test with CPG as inducer is feasible to screen the platelet dysfunction of single-donor plateletpheresis. The LG-PABER aggregometer method is more effective than SPAT.

关 键 词：单采血小板献血者 血小板功能不良 阳离子没食子酸丙酯 

分 类 号：R331.43[医药卫生—人体生理学] R193.3[医药卫生—基础医学]