RP-HPLC测定血清中氧氟沙星的浓度  

Determination of Levofloxacin in serum by RP-HPLC

作　　者：郝晓花[1] 蒋晔[1] 谢赞[1] 刘红菊[1] 郝福[1] 

机构地区：[1]河北医科大学药学院,河北石家庄050017

出　　处：《华西药学杂志》2006年第2期192-193,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的建立测定血清中氧氟沙星浓度的方法。方法采用反相液相色谱-荧光法。固定相为Phenomenex C18柱（5μm，4.6mm×250mm）；流动相为甲醇-0．05mol·L^-1磷酸二氢钠溶液、pH2．3（35：65）；检测波长λex=298nm，λem=491nm；流速1．0ml·min^-1。结果氧氟沙星的线性范围为0．037—4．064μg·ml^-1（r=0．9997）；方法回收率及提取回收率分别为97．3％和92.4％；其日内精密度RSD〈3．2％，日间精密度RSD〈5．4％。结论所用方法准确、简便、重复性好，适用于生物样品中氧氟沙星的浓度测定。OBJECTIVE To determine the concentration of Levofloxacin in serum by reversed - phase high performance liquid chromatography.METHODS The separation was performed on Phenomenex C18 column（5μm,4.6mm×250 mm） with the mobile phase consisted of methanol - sodium dihydrogen phosphate buffer（adjusting to pH2.3）（35：65）. The flow rate was 1.0 ml·min^-1. The detective wavelength was λex= 298 nm and λem = 491 nm. RESULTS The linear range for Levofloxacin in serum was 0.037 - 4.064μg·ml^-1 （ r = 0.9997）. The average extraction recovery was 92.4%. The average method recovery was 97.3% .The intra- day RSD was less than 3.2% and inter- day RSD was less than 5.4%. CONCLUSION This method is simple,rapid,accurate and reproducible. It can be used for determination of Levotloxacin in serum.

关 键 词：反相高效液相色谱法 氧氟沙星 血药浓度 

分 类 号：R917[医药卫生—药物分析学]