实验性动脉粥样硬化家兔心肌胶原的动态变化  

Dynamic changes in myocardial collagen expression in rabbits developed atherosclerosis

作　　者：贾琴[1] 常青[1] 唐海兰[1] 黄华梅[1] 李自成[2] 

机构地区：[1]暨南大学医学院组织学与胚胎学教研室,广州510632 [2]暨南大学附属第一医院心内科,广州510630

出　　处：《广东医学》2006年第4期456-457,共2页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30240076)

摘　　要：目的 观察家兔实验性动脉粥样硬化（atherosclerosis，AS）形成过程中心肌Ⅰ，Ⅲ型胶原的动态变化。方法 雄性新西兰家兔40只，随机等分为正常对照组（C组）和AS模型组（AS组）。分别于实验的第4，8，12周末随机处死每组家兔各5～8只，定位取带有冠状动脉主干及分支的心脏组织；常规石蜡切片，行HE染色及天狼猩红染色；偏光镜下观察胶原成分的分布及其量的变化。结果 与C组相比，4周末时．AS组兔心肌细胞间隙和心肌间小血管周围两型胶原已见增多、增粗；8周末以后，增加更加明显，尤其是Ⅰ型胶原。结论 AS形成过程中，心肌胶原呈持续增高趋势，且以Ⅰ型胶原为主。Objective To study the dynamic changes of type Ⅰ and Ⅲ collagen of myocardium interstitium in rabbits developed atherosclerosis. Methods 40 male New Zealand rabbits were equally randomly divided into 2 groups： control group （C group） and atherosclerosis model group （AS group）. At the 4th, 8th, 12th weekends of the experiment, 5 - 8 animals in each group were sacrificed, and cardiac tissue with coronary arteries were harvested for paraffin sections. Sections were stained with hematoxylin - eosin and picro - sirinsred separately. The content and distribution of type Ⅰ and Ⅲ collagen were examined with polarization microscopy. Results Comparing with the C group, the content of beth types of collagen in AS group significantly increased in the interstitial tissue at 4^th weekend; after 8^th weekend, this phenomenon became more serious, especially for type Ⅰ collagen. Conclusion The content of both types of collagen tends to increase persistently as athrosclerosis develops. The increase of type Ⅰ collagen is more distinct than that of type Ⅲ collagen.

关 键 词：动脉粥样硬化 心肌 胶原 显微镜检查 偏振 

分 类 号：R543.5[医药卫生—心血管疾病] R542.22[医药卫生—内科学]