应用分子印迹-固相萃取法提取中药活性成分非瑟酮  

Solid-phase Extraction of Active Component of Chinese Traditional Medicine Fisetin by Using Molecularly Imprinted Polymers

作　　者：李礼[1] 胡树国[1] 何锡文[1] 李文友[1] 陈朗星[1] 张玉奎[1] 

机构地区：[1]南开大学化学学院,天津300071

出　　处：《高等学校化学学报》2006年第4期608-611,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20375017;20375019和20445001);天津市自然科学基金(批准号:033603511)资助

摘　　要：分别以中药黄栌的主要成分非瑟酮为印迹分子、丙烯酰胺为功能单体及乙二醇二甲基丙烯酸酯为交联剂,通过封管聚合法合成了分子印迹聚合物;将其装于自制的固相萃取柱中,研究了以不同体积比的乙醇-水溶液为溶剂时非瑟酮在柱上的保留行为;通过优化清洗及洗脱条件,使非瑟酮与它的结构相似物槲皮素在柱上得到了很好的分离.Fisetin is the main function integrant in Cotinus coggygria scop of Chinese traditional medicine. Using fisetin as imprinted molecule, acrylamide as the functional monomer, and EDMA as the crosslinking agent, a polymer was prepared by molecular imprinting technique. The polymer was loaded into the self-made solid-phase extraction （SPE） cartridge. A SPE procedure was suggested for extracting and concentrating fisetin by using different volume fractions of ethanol in aqueous solution as the loading conditions. In order to optimize the washing and eluting solution, the adsorption of fisetin in different solutions was studied. It was found that the fisetin and analogue quercetin were separated primely.

关 键 词：非瑟酮 槲皮素 分子印迹聚合物(MIPs) 固相萃取 中药 

分 类 号：O657.3[理学—分析化学]