纳米脂质体包裹荧光试剂进入单细胞的研究  

Intracellular Delivery of Fluorescent Dyes Mediated by Nanometer-Liposomes

作　　者：孙悦[1] 卢敏[2] 殷学锋[1] 

机构地区：[1]浙江大学化学系微分析系统研究所 [2]浙江大学生命科学学院生物大分子与酶工程研究所,杭州310027

出　　处：《高等学校化学学报》2006年第4期632-634,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20475049)资助

摘　　要：A new method that nanometer-liposomes could penetrate the cell membrane to deliver their encapsulated material into cells was investigated.Nanometer sized-liposomes were produced from phosphatidylcholine to encapsulate fluorescent dyes and FITC labeled superoxide dismutase(SOD-FITC) by an ultrasonic method.The size distribution of liposomes was determined with a Laser Particle Analyser.The fluorescence images demonstrate that liposomes can transfer fluorescent dyes(FITC,Rhodamine B),which are not cell membrane permeable,into cells.Single cell analysis of the delivered SOD-FITC with microchip electrophoresis revealed that the density of liposomes and interaction-time between liposomes and cells affected the efficiency of intracellular delivery of encapsulated material.A new method that nanometer-liposomes could penetrate the cell membrane to deliver their encapsulated material into cells was investigated. Nanometer sized-liposomes were produced from phosphatidylcholine to encapsulate fluorescent dyes and FITC labeled superoxide dismutase （SOD-FITC） by an ultrasonic method. The size distribution of liposomes was determined with a Laser Particle Analyser. The fluorescence images demonstrate that liposomes can transfer fluorescent dyes （FITC, Rhodamine B）, which are not cell membrane permeable, into cells. Single cell analysis of the delivered SOD-FITC with microchip electrophoresis revealed that the density of liposomes and interaction-time between liposomes and cells affected the efficiency of intracellular delivery of encapsulated material.

关 键 词：单细胞分析 脂质体 细胞内衍生 荧光染料 

分 类 号：O657[理学—分析化学]