hMTH1c.83及hOGG1c.326和hMYHc.335基因多态性与慢性苯中毒风险性的关系  

Relationship between genetic polymorphism in hMTH1c.83,hOGG1c.326 and hMYHc.335 and risks of chronic benzene poisoning

作　　者：张忠彬[1] 刘薇薇[2] 顾寿永[1] 万俊香[1] 金锡鹏[1] 夏昭林[1] 

机构地区：[1]复旦大学公共卫生学院,上海200032 [2]广州市职业病防治院

出　　处：《中华劳动卫生职业病杂志》2006年第3期134-138,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目(30271113);国家973项目(2002CB512902)

摘　　要：目的探讨hMTH1Val83Met、hOGG1Ser326Cys和hMYHHis335Gln基因多态性与慢性苯中毒发病风险的关系。方法采用病例对照设计,以152名苯中毒工人为病例组,152名接触苯而没有中毒表现的工人为对照组。应用聚合酶链反应-限制性片断长度多态性分析技术(PCRRFLP)检测hMTH1c.83、hOGG1c.326和hMYHc.335位点的多态性。结果携带hMTH1c.83ValMet+MetMet和hOGG1c.326CysCys基因型的个体发生慢性苯中毒的风险性分别是携带hMTH1c.83ValVal和hOGG1c.326SerCys+SerSer基因型个体的2.51倍(ORadj=2.51,95%CI:1.14～5.49,P=0.02)和2.49倍(ORadj=2.49,95%CI:1.52～4.07,P<0.01);相比于同时携带hOGG1c.326CysCys和hMYHc.335HisHis基因型的个体,同时携带hOGG1c.326SerCys+SerSer和hMYHc.335HisGln+GlnGln两种基因型的个体有较低的慢性苯中毒发病风险(ORadj=0.33,95%CI=0.15～0.72,P=0.01);在经常吸烟的人群中,携带hMYHc.335HisGln+GlnGln基因型的个体慢性苯中毒的发病风险较携带hMYHc.335HisHis基因型的个体降低(ORadj=0.15,95%CI:0.03～0.68,P=0.01)。结论hMTH1Val83Met和hOGG1Ser326Cys多态可能与个体慢性苯中毒的发病风险改变有关,而hOGG1Ser326Cys和hMYHHis335Gln基因多态之间可能存在潜在的联合作用。Objective To explore the relationship between genetic polymorphisms in hMTH1, hOGG1 and hMYH and risks of chronic benzene poisoning（CBP）. Methods A case control study was conducted. One hundred and fifty- two BP patients and 152 workers occupationally exposed to benzene without poisoning manifestations were investigated, The polymerase chain reaction restrainedfragment length polymorphism technique （PCR-RFLP） was applied to detect the single nucleotide polymorphisms（SNPs） on c. 83 of hMTItl gene, c. 326 of hOGGI gene and c.335 of hMYH gene. Results There were2.51 times（ORadj =2.51,95%CI：1.14-5.49,P=0.02） and 2.49 times（ ORadj = 2.49, 95% CI： 1.52 - 4.07, P 〈 0.01 ） risks of BP for individuals carrying genotypes of hMTH1c. 83Val/Met ＋ Met/Met or hOGG1e. 326Cys/Cys compared with individuals carrying genotypes of hMTH1 c. 83Val/Val or hOGG1c. 326Ser/Cys ＋ Ser/Ser, respectively. Compared with individuals carrying genotypes of hOGGlc. 326Cys/Cy and hMYHc .335is/His at the same time,there was 0.33 times（ ORadj = 0.33,95% CI = 0.25 - 0.72, P = 0.02） risks of BP for these with genotypes of hOGGlc. 326Ser/Cys ＋ Ser/Ser and bMYHc.335His/Gln＋ Gin/Gin simultaneously. In the smoking group, there was 0. 15 times（ ORadj = 0.25,95% CI： 0.03 - 0.68, P = 0.01） risks of BP for subjects carrying genotypes of bMYHc. 335His/Gln ＋ Gln/Gln compared with thee carrying genotypes of hMYHc. 335His/His. Conclusion Polymorphisms of hMTH1 Va/83Met and hOGG1 Ser326Cys may contribute to altered risks of CBP, and potential interaction may exist among polymorphisms of hOGG1 Ser326Cys and hMYH His335Gln.

关 键 词：苯中毒 基因hMTH1 HOGG1 hMYH 基因多态性 

分 类 号：R135[医药卫生—劳动卫生]