α黑素细胞刺激素基因转染对小鼠骨髓来源的树突状细胞成熟的影响  

Effect of the α-melanocyte stimulating hormone gene transfection on the maturation of the mouse bone marrow-derived dendritic cells

作　　者：张丽娟[1] 田野苹[1] 

机构地区：[1]第二军医大学免疫学研究所

出　　处：《现代免疫学》2006年第2期102-107,共6页Current Immunology

基　　金：上海市科委基础研究重点资助项目(02JC14027)

摘　　要：为观察转染α黑素细胞刺激素(-αMSH)基因的树突状细胞(DC)的功能和特性,以腺相关病毒(AAV)为载体将α-MSH基因导入小鼠骨髓来源的未成熟DC(-αMSH-DC),用ELISA检测-αMSH-DC培养上清中-αMSH及IL-12水平,用流式细胞仪分析-αMSH-DC表面分子的表达,以-αMSH-DC提呈OVA抗原刺激致敏T细胞,通过ELISA测定IL-2水平来观察其抗原提呈功能。结果显示,在-αMSH-DC培养上清中检测到-αMSH的分泌,-αMSH-DC表面分子表达下调,分泌IL-12的能力降低,抗原提呈功能被抑制。-αMSH-DC可部分抵抗LPS上调表面分子表达和促进IL-12分泌的作用。表明-αMSH基因可籍AAV载体导入未成熟DC,并有效地表达,α-MSH基因修饰的DC成熟受到抑制。To investigate the functions and characteristics of the dendritic cells transfeeted by α-melanoeyte stimulating hormone gene （α-MSH-DC）,α-MSH gene was transferred into the mouse bone marrow-derived immature dendritic cells by adeno-assoelated virus vector （AAV）. The levels of α-MSH and IL-12 in the supernatant of α-MSH DC culture were determined with EIASA. Using FACS, the phenotypic characteristics of a-MSH-DC were analyzed. The antigen presenting capacity of α-MSH DC was determined by detecting the IL-2 secretion of OVA sensitized T lymphocytes stimulated with α-MSH DC and OVA in vitro. The results showed that α-MSH was detectable in the α-MSH DC culture supernatant. The expression of surface mole cules on α-MSH-DC was down-regulated and the secretion of IL-12 by α-MSH-DC was reduced. The antigen presenting capaci ty of （α-MSH DC was inhibited, α-MSH-DC could partly resist the effects of LPS in up regulating the surface molecule expresL sion and promoting the IL-12 secretion. It is concluded that by AAV vector, α-MSH gene can be transferred into the mouse immature dendritic cells and express availably, while the maturation of α-MSH gene modified DC was suppressed.

关 键 词：Α黑素细胞刺激素 腺相关病毒 基因修饰 树突状细胞 

分 类 号：R392.1[医药卫生—免疫学]