原发性胆汁肝硬化患者血清中抗线粒体抗体及其亚型和抗核抗体的联合检测的临床价值  

Detection of the anti-mitochondrial antibody,its subtypes and the anti-nuclear in sera of patients with primary biliary cirrhosis

作　　者：朱烨[1] 屠小卿[1] 周琳[1] 仲人前[1] 孔宪涛[1] 

机构地区：[1]第二军医大学长征医院,上海200003

出　　处：《现代免疫学》2006年第2期136-138,共3页Current Immunology

基　　金：国家自然科学基金资助项目(30080027);上海市基础研究重大资助项目(02JC14005);上海市百人计划基金资助项目(沪卫9713)

摘　　要：为了分析原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)患者的抗线粒体抗体(anti-mitochondria antibody,AMA)及其M2、M4、M9亚型、抗核抗体(antinuclear antibody,ANA)的阳性率。应用间接免疫荧光法检测ANA、AMA,免疫斑点法检测AMA M2、M4、M9。结果显示91例PBC患者中AMA有89例为阳性。其中96.7%(88/91)的患者M2型阳性,45.1%(41/91)M4型阳性,2.2%(2/91)M9型阳性。39/91例患者ANA阳性,其中21例为核膜型。ANA、AMA及其M2、M4、M9亚型联合检测对于PBC患者的诊断有重要价值。The anti-mitochondrial antibody（AMA） and the anti nuclear antibody（ANA） in sera of 91 patients with primary biliary cirrhosis（PBC） were detected by indirect immunofluorescence assay, and the immunoblot assay was used to detect the subtypes of AMA. It was found that in the sera of these patients, 97.8%（89/91） of patients with PBC was positive for AMA, in which 96.7%（88/91）, 45.1%（4/91） and 2.2%（2/91） were positive for subtype M2, M4 and M9 of AMA respectively. Thirty nine patients out of 91 patients with PBC（42.9% ） were positive for ANA, in which 21 patients showed nuclear membranous pattern. It is concluded the combined deteetioin of AMA, its subtypes and ANA may be important for the diagnosis of primary biliary cirrhosis.

关 键 词：原发性胆汁性肝硬化 抗线粒体抗体 抗核抗体 

分 类 号：R575[医药卫生—消化系统]