福尔马林致痛大鼠背根节内5-HT受体亚型mRNA的表达变化  

CHANGES IN 5-HT RECEPTOR SUBTYPES mRNA EXPRESSION IN THE DORSAL ROOT GANGLIA IN RATS WITH FORMALIN-INDUCED INFLAMMATORY PAIN

作　　者：刘翔宇[1] 武胜昔[1] 王亚云[1] 王文[1] 周亮[1] 黄静[1] 李云庆[1] 

机构地区：[1]第四军医大学基础医学部解剖学教研室梁銶琚脑研究中心,西安710032

出　　处：《中国疼痛医学杂志》2006年第1期28-31,共4页Chinese Journal of Pain Medicine

基　　金：国家重点基础研究规划"脑功能的动态平衡调控"(G2006CB500808);陕西省自然科学基金(2004C2-24)资助项目

摘　　要：目的：探讨外周5-HT受体亚型在外周伤害性感受中的作用.方法：用反转录PCR技术观察大鼠单侧足底皮下注射福尔马林致痛后背根节内5-HT1～7受体亚型mRNAs的表达变化.结果：在大鼠足底皮下注射福尔马林后1 h,注射侧腰段背根节内5-HT1A、5-HT1B、5-HT2A、5-HT3、5-HT4和5-HT7受体亚型mRNAs的表达水平显著升高,而5-HT1D、5-HT1F、5-HT2C、5-HT5A和5-HT6受体亚型mRNAs的表达在福尔马林致痛后无明显变化.在正常和福尔马林致痛大鼠的背根节内均未检测到5-HT1E、5-HT2B和5-HT5B受体亚型mRNAs的表达.结论：5-HT1A、5-HT1B、5-HT2A、5-HT3、5-HT4和5-HT7受体亚型可能参与了福尔马林诱导的炎性痛,且它们在外周伤害性信息的传递方面可能发挥不同的作用.Objective： To investigate the involvement of 5-HT receptors in the peripheral nociceptive signaling. Methods： The expression of 5-HT receptor subtype mRNAs was detected in the lumbar dorsal root ganglion （DRG） by reverse transcription-polymerase chain reaction （RT-PCR） following unilateral injection of formalin into the plantar surface of rat hind paw. Results： Formalin administration resulted in a significant increase in the mRNA level of 5-HT1A, 5-HT1B, 5-HT2A, 5-HT3 , 5-HT4 and 5-HT7 receptor subtypes in the ipsilateral DRG at 1 h, but not at 10 min or 3 h after formalin injection. Expression of 5- HTID, 5-HT1F, 5-HT2c, 5-HT5A and 5-HT6 receptor subtype mRNAs remained no remarkable changes at these time points. 5-HT1E, 5-HT2B and 5-HT5B receptor mRNAs were not detectable in the DRG in both normal and formalin rats. Conclusion： The present results suggest that 5-HT1A, 5-HT1B, 5-HT2A, 5-HT3, 5-HT4 and 5-HT7 receptor subtypes might be involved in the formalin-induced inflammatory pain. Different change patterns of 5-HT receptor subtypes in rat DRG following inflammatory pain indicate the possible different roles of each 5-HT receptor subtype in the transmission of peripheral nociception.

关 键 词：5-HT受体 背根神经节 反转录PCR 大鼠 

分 类 号：R402[医药卫生—临床医学]