铅中毒对生长发育期小鼠学习记忆能力的影响  

Effects of lead poisoning on learning and memory performances in mice at the phase of growth and development

作　　者：何瑞芳[1] 张艳[2] 杨艳旭[3] 张利彬[1] 李东亮[1] 杜爱林[1] 

机构地区：[1]新乡医学院生理学与神经生物学教研室,河南省新乡市453003 [2]新乡医学院机能学研究室,河南省新乡市453003 [3]新乡医学院公共卫生学系,河南省新乡市453003

出　　处：《中国临床康复》2006年第14期62-64,共3页Chinese Journal of Clinical Rehabilitation

基　　金：河南省自然科学基金资助项目(0111020300)~~

摘　　要：目的:观察不同量铅中毒对生长发育期小鼠学习记忆能力的影响。方法:实验于2005-10/11在新乡医学院生理学与神经生物学实验室。选取21d健康断乳的昆明小鼠40只,按随机数字表法随机分为4组,蒸馏水阴性对照组,10mg/kg染铅组、20mg/kg染铅组、40mg/kg染铅组,每组10只,雌雄各半,适应性饲养。每天8:00～17:00对小鼠采用水迷宫进行学习记忆训练及测试,第1天训练从离台阶较近的出口处有1个盲端处开始,不用的盲端用挡板挡住,第2,3,4天的训练加长路程,均从离台阶较远的出口处有3个盲端处开始,若在120s内找不到台阶,则将小鼠引导到台阶上并保持10s,每只小鼠每天训练2次,以第2次训练成绩为准。4d训练结束后,10mg/kg染铅组、20mg/kg染铅组、40mg/kg染铅组小鼠分别予以1g/L,2g/L,4g/L醋酸铅10mL/kg灌胃,1次/d,连续33d,造成慢性铅中毒。在染毒7d后,进行为期4d的第一次水迷宫测试,测试点与训练时相同;此后一边继续染毒一边测试,连续染毒18d后行第2次测试,连续染毒29d后行第3次测试,此时小鼠鼠龄58d,已基本发育成熟。结果:实验中动物无死亡、丢失情况。均进入结果分析。①小鼠水迷宫训练4d后,各组之间各点测试成绩(错误次数和反应时间)的差异无显著性意义。②第1次水迷宫测试,40mg/kg染铅组小鼠在第1天反应时间与第4天的错误次数显著高于对照组,差异有显著性意义(P<0.05)。③第2次水迷宫测试,40mg/kg染铅组第1天的反应时间、第2天的错误次数与第3,4天的错误次数和反应时间均显著高于对照组,差异有显著性意义(P均<0.05),而10mg/kg染铅组、20mg/kg染铅组的测试成绩与对照组之间的差异无显著性意义。④第3次水迷宫测试,40mg/kg染铅组第3天错误次数与第1,2,4天的错误次数和反应时间均显著高于对照组,差异有显著性意义(P均<0.05),而10mg/kg染铅组、20mg/kg染铅组的测试成绩与对照组之间的AIM： To observe the effect of .lead poisoning of different content on the learning and memory performances of mice on the stage of growth and development. METHODS： The experiment was carried out in the Laboratory of Physiology and Neurobiology, Xinxiang Medical College between October and November 2005. Forty healthy 21-day-old Kunming mice were randomly divided into 4 groups with 10 mice （5 males and 5 females） in each group： distilled water negative control group, 10, 20 and 40 mg/kg lead poisoning groups. The mice were adaptively fed. The water-maze was adopted to train and test the learning and memory every day at 8：00-17：00, For thee first day, the training began from a blind point nearer to the platform, and other not needed blind points were blocked up with blind, while for the second, third and fourth days, the distance was prolonged, and the training began from three blind points far from the platform. If the trained mouse did not find the platform for t20 seconds, then the mouse was led to the platform and retained for 10 seconds. Each mouse was trained for twice every day, and the mouse behavior of the second time was recorded. After the 4-day training, the mice in the 10, 20 and 40 mg/kg lead poisoning groups were given gastric peffusion of 1, 4 and 4 g/L lead acetate （10 mL/kg）, respectively, once a day for 33 days continuously, to induce chronic lead poisoning. At 7 days after poisoning, the 4-day water maze test was given for the first time, the tested points were the same as those in the training; and then the mice were tested while poisoning, the second and third tests were given at 18 and 29 days after the continuous poisoning, when the mice were 58 days old and had grown up. RESULTS： There was no death or loss of mice during the experiments, and all the mice were involved in the analysis of results. （1） After the 4-day training, there were no significant differences in the tested results （error times and reaction time） among the groups. （2） For the first test

关 键 词：学习 记忆 铅中毒 鼠 

分 类 号：R338.64[医药卫生—人体生理学]