饥饿状态下大鼠胰岛α细胞免疫细胞化学及形态计量研究  

Immunocytochemical and Stereological Research of α Cells in the Islet of Fasting Rat

作　　者：王小丽[1] 张亦农[1] 刘胜洪[1] 康学军 王庆堂[1] 

机构地区：[1]武汉同济医科大学基础医学院组织胚胎学教研室

出　　处：《同济医科大学学报》1996年第2期100-103,共4页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金

摘　　要：应用免疫细胞化学方法对饥饿５ｄ大鼠和正常大鼠胰鸟α细胞进行显示，并用显微分光光度计和体视学技术对２组动物α细胞内胰高糖素的反应强度和α细胞在胰岛内所占数量和体积进行计量分析。结果表明：与正常对照组相比，饥饿大鼠胰岛α细胞内胰高糖素含量明显下降，但α细胞在胰岛内的相对数量和体积并无明显变化。提示：饥饿可导致胰高糖素释放，但由于大鼠胰岛α细胞在胰岛内并未出现相应数量和体积增加的代偿性改变，因此推测在持续性饥饿过程中α细胞对血糖不起主要调节作用。In this study,the a cells of pancreas islets from fasting rats and normal rats were demonstrated with methods of immunocytochemistry,and the optic density of glucagon immunocytochemical reaction within the cells was quantitated by microspectrophotometer.The volume density and numerical density of the α cells were also measured by stereological method.The results showed that the glucagon immunocytochemical reaction within the α cells of fasting rats was significantly decreased as compared with that of normal rats,but no marked difference of the volume density and numerical density of the a cells was found between the two groups.This study suggested that the α cells of pancreas islets can be stimulated by fasting to release glucagon,but they may not play an impotant role in the regulation of blood glucose during prolonged fast because no corresponding changes of increasing the related volume and number of the α cells occurred in the pancreas islets of fasting rats.

关 键 词：大鼠 胰岛Α细胞 体视学 免疫细胞化学 

分 类 号：Q959.837[生物学—动物学] R392.12[医药卫生—免疫学]