镁抗大鼠再灌注心律失常作用与心肌代谢的相互关系  

Interrelationship between Protective Effect of Magnesium on Reperfusion Arrhythmias and Metabolic Substrates

作　　者：向世勤[1] 赵华月[1] 

机构地区：[1]武汉同济医科大学附属同济医院内科

出　　处：《同济医科大学学报》1996年第2期111-114,共4页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金

摘　　要：根据３种不同Ｍｇ￣（２＋）浓度的灌流液将３６只大鼠随机均分为３组。采用Ｌａｎｇｅｎｄｏｒｆｆ装置灌注离体心脏模型，研究不同浓度Ｍｇ￣（２＋）灌流时，再灌注心律失常（ＲＡ）发生的情况，同时检测再灌注前、后冠脉流出液中环磷酸腺苷（ｃＡＭＰ）、血栓素Ｂ_２（ＴＸＢ_２）和６－酮－前列腺素Ｆ_（１α）（６－Ｋ－ＰＧＦ_（１α））浓度变化，并探讨其相互关系。结果显示，镁可有效地降低ＲＡ发生率，同时减少再灌注时ｃＡＭＰ、ＴＸＢ_２和６－Ｋ－ＰＧＦ_（１α）的合成及释放。当镁分别为０．４、１．２或４．８ｍｍｏｌ／Ｌ时，再灌注心室颤动分别为１００％、３３．４％和１６．７％（均为Ｐ＜０．０１）。而且再灌注后３种代谢产物增加百分率也显著下降，其中ｃＡＭＰ分别为１１８％、６７％和３２％（均为Ｐ＜０．０１）；ＴＸＢ_２为１６１％、１０６％（Ｐ＜０．０５）和２８％（Ｐ＜０．０１）；６－Ｋ－ＰＧＦ_（１α）为５７％、４４％（Ｐ＜０．０５）和１９％（Ｐ＜０．０１）。结果提示，镁可以显著降低再灌注心肌细胞合成及释放ｃＡＭＰ、ＴＸＡ_２和ＰＧＩ_２，因而降低ＲＡ发生率。rats were randomly equally divided into 3 groups according to high,medium and low concentration of Mg￣(2+)in perfusate. The isolated Langendorff-perfused rat heart model was used to evaluate the effect of different concentrations of Mg￣(2+)on the interrelationship of the incidence of reperfusion arrhythmias(RA)and changes of cAMP,TXB_2 and 6-K-PGF_(1α),measured from coronary effluent before and after reperfusion.The result showed that Mg￣(2+)has beneficial effect on reducing RA,meanwhile it can reduce the synthesis and release of cAMP,TXB_2 and 6-K -PGF_(1α) on reperfusion.When Mg￣(2+)was 0.4,1.2 and 4.8 mmol/L respectively, reperfusion ventricular fibrillations were 100%,33.3%(P<0.01)and 16.7%(P<0.01)respectively.The percentage of increase of the three metabolic substrates reduced significantly,of which cAMP were 118%,67%(P<0.01)and 32%(P<0.01),TXB_2 161%,106%(P<0.05),28%(P<0.01)and 6-K-PGF_(1α)57%,44%(P<0.05),19%(P<0.01)repectively.This suggests that magnesium can markedly reduce the synthesis and release of cAMP,TXA_2 and PGI_2 in reperfused myocardial cells,thus alleviating the incidence of RA.

关 键 词：再灌注 心律失常 镁 环磷酸腺苷 TXB2 心肌代谢 

分 类 号：R541.702[医药卫生—心血管疾病]