半夏泻心方及其配伍药组含药血清对人胃癌BGC-823细胞增殖及bcl-2表达的影响  

The Effect of the Components in Serum of Banxiaxiexin Prescription and Different Herbal Fomulation on the Cell Multiplication of BGC-823 and Expression of bcl-2 in Human Gastric Cancer

作　　者：席时燕[1] 刘喜平[1] 李沛清[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000

出　　处：《中国中医药信息杂志》2006年第4期20-22,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：甘肃省教育厅基金资助项目(01B-32)

摘　　要：目的研究半夏泻心方不同配伍药组含药血清对BGC-823细胞凋亡的影响及其机制。方法采用MTT法观察半夏泻心方配伍药组含药血清对BGC-823细胞增殖的影响,S-P免疫组化法检测凋亡相关基因bcl-2的表达。结果半夏泻心方配伍中辛开组、苦降组和由其组成的辛苦组可明显抑制BGC-823细胞的生长,显著高于辛甘组、苦甘组、甘补组和全方组(P<0.05),呈剂量-效应关系,同时可下调bcl-2的表达。结论半夏泻心方中辛开、苦降的配伍形式可抑制BGC-823细胞增殖,其诱导细胞凋亡作用与下调bcl-2有关。辛开、苦降配伍后(辛苦)有显著的协同增效趋势,而其它配伍药组可能存在拮抗作用。提示辛开苦降法可能是中医药防治胃癌的主要治则治法之一。Objective To study the apoptosis in human gastric cancer cell BGC-823 induced by the components in serum of Banxia Xiexin prescription and different herbal fomulation. Method multiplication of cell BGC-823 was observed with MTT method. The genic expression of bcl-2 was tested by S-P immunohistochemistry tehnique. Results As for inhibition of cell BGC-823 multiplication, the groups of Xinkal, Kujiang and Xinloa in Banxia Xiexin prescription and different herbal fomulation was dose-dependent and more sensitive than the groups of Xingan, Kugan, Ganbu and the intact prescription （P〈0.05）. Moreover, there was a positve relationship in ganic expression of bcl-2. Conclusion The groups of Xinkal and Kujiang in Banxia Xiexin prescription could inhibit multiplication of cell BGC-823. The apoptosis induced by Xinkal and Kujiang was linked with bcl-2. It was positive cooperative during the compatibility of Xinkal and Kujiang. However, it was antagonistic during others compatibility. It suggested that the way of Xinkal and Kujiang is important for cure and prevention of gastric cancer.

关 键 词：半夏泻心方 方剂配伍 细胞凋亡 

分 类 号：R289.11[医药卫生—方剂学]