高效液相色谱法测定轻身减肥片中原儿茶醛含量  

Content Determination of Protocatechuic Aldehyde in Qingshenjianfei Tablets by HPLC

作　　者：何柏良 李战 

机构地区：[1]广西玉林制药有限责任公司,广西玉林537001

出　　处：《中国中医药信息杂志》2006年第4期44-45,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立轻身减肥片中原儿茶醛的含量测定方法。方法采用高效液相色谱法,HypersilC18柱(4.6mm×250mm,5μm);流动相:甲醇-0.5%冰醋酸(12∶88);检测波长280nm。结果原儿茶醛线性范围为0.05～0.60μg,平均加样回收率为100.49%,RSD=1.61%。结论方法可靠,简单可行,为控制轻身减肥片的内在质量提供了科学依据。Objective To set up a method for determining the protocatechuic aldehyde in Qingshenjianfei tablets. Method HPLC method, Elite Hypcrsil C18 column （4.6 mm×250 mm, 5μm） was used, mobile phase： Methanol-0.5% Acetic Acid Glacial （12 ： 88）, the detecting wavelength was at 280 nm. Result The standard curve was linear within a range of 0.05-0.60 μg, The average recovery and RSD were 100.49% and 1.61%. Conclusion This method is accurate, reliable, and can provide a scientific index for quality control of Qingshenjianfei Tablets.

关 键 词：轻身减肥片 原儿茶醛 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]