双歧杆菌的完整肽聚糖对巨噬细胞NF-kB的影响  

Influence of whole peptidoglycan of Bifidobacteria on NF-kB of macrophages

作　　者：朱忠生 王立生 杨林 朱惠明 赖卓胜[2] 姜泊[2] 潘令嘉[2] 周殿元[2] 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院消化科,广东深圳518020 [2]南方医科大学南方医院消化科,广东广州510515

出　　处：《中国微生态学杂志》2006年第2期83-85,共3页Chinese Journal of Microecology

基　　金：广东省自然基金资助课题;编号:0400696105009077

摘　　要：目的从NF-kB角度探索分叉双歧杆菌的完整肽聚糖(WPG)激活巨噬细胞的信号机制。方法首先分离培养SD大鼠腹腔巨噬细胞,然后以不同浓度的WPG刺激巨噬细胞,最后采用凝胶电泳迁移分析技术检测巨噬细胞NF-kB的DNA结合活性。结果分叉双歧杆菌的WPG作用于巨噬细胞后,其NF-kB的DNA结合活性明显高于对照组(P<0.01)。结论分叉双歧杆菌的WPG能活化巨噬细胞的NF-kB。Objective To explore the signal mechanisms of whole peptidoglycan （WPG） of Bifidobacteria bifidum activating macrophages in view of NF-kB. Methods SD rat peritoneal macrophages rats were separated, and cultivated. The macrophages were stimulated by WPG with different concentration. NF-kB DNA binding activity of macrophages was detected by using electrophoretic mobility shift assay. Result NF-kB DNA binding activity of the macrophages in experimental group was significantly higher than that in control group after the macrophages were stimulated by WPG （ P 〈 0.01 ）. Conclusion WPG of Bifidobacteria bifidum could activate NF-kB of macrophages.

关 键 词：双歧杆菌 完整肽聚糖 巨噬细胞 核因子KB 

分 类 号：R378.992[医药卫生—病原生物学]