普通鵟(Buteo buteo)消化系统各器官蛋白水解酶种类和活性分析  

ANALYSIS OF KINDS AND ACTIVITY OF PROTEOLYTIC ENZYMES IN DIGESTIVE SYSTEM OF BUTEO BUTEO

作　　者：牛红星[1] 卜艳珍[1] 余燕[1] 王艳酶[1] 姬生栋[1] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,新乡453007

出　　处：《解剖学报》2006年第2期177-180,共4页Acta Anatomica Sinica

基　　金：河南省自然科学基金资助项目(0411032500);河南省动物学省级重点学科经费资助

摘　　要：目的研究普通(Buteo buteo)消化系统各器官蛋白水解酶的种类和性质,为探讨野生鸟类的分类地位、系统演化提供资料。方法采用蛋白水解酶复性电泳(G-PAGE)技术。结果1.普通消化系统蛋白水解酶种类多,达26种;2.普通消化系统蛋白水解酶的活性受pH值的影响和制约,在碱性条件下酶活性最强,酸性条件下最弱,酶活性表现为碱性>中性>酸性;3.不同pH条件下,十二指肠和胰蛋白水解酶种类多且活性强,舌、食道和嗉囊蛋白水解酶种类少且活性弱;4.不同pH条件下,75、70 kD酶带普遍存在于肝脏和胰脏以外的各消化器官中。结论消化系统中结构相似、功能相近的各器官之间,蛋白水解酶的种类和性质相似。75、70 kD蛋白水解酶的pH依赖性不强且分布广。198 kD蛋白水解酶在pH8.5时广泛存在于消化系统各器官。Objective The kinds and activities of proteolytic enzymes of tongue, esophagus, craw, muscular stomach, glandular stomach, duodenum, large intestine, pancreas, liver in digestive system of Buteo buteo were studied to provide basic information for taxonomy and evolvement study of wild birds. Methods The experiment was carried out at 4℃ by using gelatine polyacrylamide gel electrophoresis（G-PAGE） ameliorated by Xu Cunshuan. Results 1. At least 26 kinds of proteolytic enzymes were found in digestive system of Buteo buteo. 2. The kinds and activities of proteolytic enzymes in the digestive system of Buteo buteo were affected by pH. The activities of proteolytic enzymes was the weakest in the acid environment and the strongest in the alkaline environment, showing that the activity of the alkaline proteolytic enzymes were stronger than that of the neutral proteolytic enzymes, and that of the acid proteolytic enzymes were weakest. 3. Duodenum and pancreas had more kinds and stronger activities of proteolytie enzymes than those in tongue, esophagus and craw in any pH value condition. 4. The proteolytic enzymes with 75 and 70 kD molecular weight existed in every organ of digestive system except pancreas and liver in any pH condition. Conclusion The similarity of the kinds and the activities of proteolytic enzymes exist in the organs with similar structure and similar functions. The proteolytic enzyme of 75 and 70 kD molecular weight, whose pH-dependent activity is not obvious, exists widely in digestive system of Buteo buteo. The activity of proteolytic enzyme with 198 kD molecular weight is found in every organ of digestive system under pH 8.5 condition.

关 键 词：蛋白水解酶 消化系统 复性电泳(G-PAGE) 普通鵟 

分 类 号：Q959.7[生物学—动物学]