Ⅰ型和Ⅳ型胶原蛋白在花背蟾蜍蝌蚪变态期角膜发育过程中的免疫组织化学研究  

IMMUNOHISTOCHEMICAL STUDY OF EXPRESSION OF TYPE Ⅰ AND TYPE Ⅳ COLLAGEN IN THE CORNEAL DEVELOPMENT OF BUFO RADDEI LARVAE DURING METAMORPHOSIS

作　　者：张丽[1] 刘怀金[2] 刘重斌[3] 王子仁[3] 

机构地区：[1]湖南理工学院化学化工系,岳阳414000 [2]甘肃农业大学动物科学技术学院,兰州730070 [3]兰州大学生命科学学院,兰州730000

出　　处：《解剖学报》2006年第2期199-202,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30170109)

摘　　要：目的探讨花背蟾蜍蝌蚪变态期角膜发育过程中Ⅰ型和Ⅳ型胶原蛋白的时空分布及其增龄变化规律。方法采用SP免疫组织化学方法分别检测花背蟾蜍蝌蚪后肢芽期、后肢发育期、前肢芽期、完全变态期、1年和3年以上成体角膜中Ⅰ型和Ⅳ型胶原蛋白的表达。结果花背蟾蜍蝌蚪在变态期间,其内、外角膜逐渐愈合,Ⅰ型胶原蛋白在此期间表达广泛,在前界层、角膜基质、后界层和角膜内皮均有表达,尤其是在角膜基质中呈渐次增多趋势;Ⅳ型胶原蛋白最初只在前界层中表达,从前肢芽期开始,除了在前界层和后界层中均有表达外,角膜基质和角膜内皮中也有逐渐增多的表达,只是表达量与Ⅰ型胶原蛋白不同。结论Ⅰ型胶原蛋白在花背蟾蜍蝌蚪变态期角膜发育过程中对角膜基质的构建和功能的维持起一定作用;Ⅳ型和Ⅰ型胶原蛋白在角膜基质中表达的差异性,表明角膜基质的构建还部分地取决于不同类型胶原蛋白适当的搭配比例。Objective To investigate the spatintemporal patterns and age-related changes of expression of type Ⅰ and type Ⅳ collagen in the corneal development of Bufo raddei larvae during metamorphosis. Methods Using immunohistochemieal method,type Ⅰ and Ⅳ collagen distributions were investigated in the cornea of Bufo raddei at hlnd-limb bud stage,hind-limb developing stage, forelimb bud stage, complete metamorphosis stage, one-year-old adult and three-year-old adult, respectively. Results The inner and outer cornea fused together into mature cornea during metamorphosis. Type Ⅰ collagen was expressed widely during the period,it existed in Bowman＇s membrane, Deseemet＇ s membrane, corneal stroma and endothelium, especially showing a progressive change in corneal stroma. The expression of type Ⅳ collagen was only observed in Bowman＇ s membrane and Deseemet＇s membrane at first,since the forelimb bud stage,it also expressed in corneal stroma and endothelium, but it didn＇t express the same as Type Ⅰ. Conclusion Type Ⅰ eollagen has the role in the eonstruetion of the eorneal stroma and its fuetion in the eorneal development of Bufo raddei larvae during metamorphosis. Type Ⅳ and type Ⅰ eollagen expressed differently in eorneal stroma showing that the eonstruction of the eorneal stroma may depend partly on the right expression proportion of different eollagen type.

关 键 词：变态期角膜发育 胶原蛋白 免疫组织化学 花背蟾蜍蝌蚪 

分 类 号：Q954.53[生物学—动物学]