神经移植术修复幼年大鼠臂丛神经损伤后对神经元保护作用的实验研究  

Nerve grafting after brachial plexus Injury in young rats:an experimental study of the protective effect on neurons

作　　者：傅阳[1] 陈亮[1] 顾玉东[1] 

机构地区：[1]复旦大学附属华山医院手外科,上海200040

出　　处：《中华手外科杂志》2006年第2期103-105,共3页Chinese Journal of Hand Surgery

基　　金：国家973项目(2003CB515305)

摘　　要：目的研究神经移植术修复幼年大鼠臂丛神经损伤后对神经元的保护作用。方法将出生18 d SD大鼠24只等分为2组。神经根切断组：将右侧颈5神经根切除0．3cm。神经根修复组：颈5神经根部分切除后取腓肠神经移植修复。采用True Blue注射法逆行标记神经元。于术后4周取颈5脊髓和背根神经节,应用TUNEL法检测运动及感觉神经元中细胞凋亡情况,并观察两组神经元数量的变化。结果与神经根切断组相比,神经根修复组神经元数量显著增加(P＜0．01),凋亡细胞数明显减少(P＜0．01)。结论神经移植术修复幼年大鼠臂丛神经损伤后对近端运动和感觉神经元有保护作用。Objective To investigate the protective effect of nerve grafting on neurons after brachial plexus nerve injury in young rats. Methods Model of nerve root resection was set up in 24 18-day-old Spragne Dawley rats. Two groups were designed in equal number. One group underwent nerve root resection, the other one had nerve grafting to repair the nerve root defect. At 4 weeks postoperatively, survived neurons and apoptosis cells were detected by using the True Blue retrograde tracing technique and the terminal deoxynucleotidyl transferase （TUNEL） method. The numbers of True Blue positively labeled survived neurons and apoptosis ceils in C5 anterior horn and dorsal root ganglia （DRG） of the two groups were observed. Results Compared with the group of C5 nerve root resection without repair, the number of True Blue positively labeled survived neurons in the group of nerve root transection and grafting was significantly higher （ P 〈 0.01 ）, and the number of apoptnsis cells was significantly lower （ P 〈 0.01 ）. Conclusion Nerve grafting has a protective effect on sensory and motor neuron survival after brachial plexus nerve injury in young rats.

关 键 词：臂丛 神经元 细胞凋亡 神经移植 

分 类 号：R651.3[医药卫生—外科学]