RP-HPLC法测定中华绒螯蟹主要组织中的恩诺沙星及其代谢产物  

Development of RP-HPLC for determination of enrofloxacin and its metabolite in tissues of Chinese mitten-handed crab(Eriocheir sinensis) by RP-HPLC

作　　者：郑宗林[1] 唐俊[1] 喻文娟[1] 胡鲲[1] 叶金明[1] 杨先乐[1] 

机构地区：[1]上海高校水产养殖E-研究院上海水产大学农业部水产种质资源与养殖生态重点开放实验室农业部渔业动植物病原库,上海200090

出　　处：《上海水产大学学报》2006年第2期156-162,共7页Journal of Shanghai Fisheries University

基　　金：科技部项目"重要技术标准研究"(2002BA906A39-01);国家科技攻关项目(2004BA526B0504);上海市重点学科建设资助项目(Y1101);上海市教育委员会E-研究院建设资助项目(E03009)

摘　　要：建立了反相高效液相色谱法(RP-HPLC)测定中华绒螯蟹肌肉、肝胰腺、精巢和卵巢组织内的恩诺沙星、环丙沙星含量。结果表明,恩诺沙星和环丙沙星在四种组织中的平均回收率分别为70.5%±12.61%,70.25%±14.73%;日内精密度分别为3.27%±0.84%,2.14%±0.68%;日间精密度分别为5.38%±2.46%,3.48%±1.16%。恩诺沙星、环丙沙星的最低检测限分别为0.02μg/g和0.004μg/g。以10 mg/kg蟹体重剂量单次肌肉注射给药后,恩诺沙星在中华绒螯蟹肌肉、肝胰腺、精巢和卵巢内的Tmax,Cmax分别为:1 h,4.323±0.56μg/g;1 h,6.042±0.72μg/g;3 h,2.381±0.43μg/g;3 h,2.101±0.29μg/g,各组织的药物消除半衰期(t1/2β)分别为:45.186 h,73.93 h,45.577 h,38.081 h。各组织中均能检测到环丙沙星,但含量均处较低水平,且代谢和消除起伏波动较大。本方法快速、灵敏、准确,适合于中华绒螯蟹组织中恩诺沙星、环丙沙星含量分析。The methods of reverse-phase high performance liquid chromatography （RP-HPLC） were developed to determine concentration of enrofloxacin（ENR） and ciprofloxacin（CIP） in muscle, hepatopancreas, spermary, ovary of Chinese mitten-handed crab（ Eriocheir sinensis ）. The average recovery of ENR and CIP in four tissues is 70.5% ±12.61%,70.25% ± 14.73%;The intra-day precision of ENR and CIP is 3.27% ± 0.84%, 2.14% ± 0.68%, respectively; The inter-day precision is 5. 38% ± 2.46%, 3. 48% ± 1. 16%, respectively. The lowest detected limit of enrofloxacin in tissues is 0.02 μg/g, and cipmfloxacin is 0. 004 μg/g. ENR was administered to E. sinensis in a single dose（ 10 mg/kg b. w. ） following intramuscular administration. The Tmax, Cmax of ENR in four tissues are 1 h,4.323 ± 0.56μg/g; lh,6.042 ± 0.72μg/g;3 h,2.381 ± 0.43 μg/g;3 h, 2.101 ± 0.29 μg/g, respectively, and the half-life of elimination in these tissues is 45.186 h, 73.93 h, 45. 577 h, 38.081 h, respectively. The method is fast, sensitive, precise and suitable to determine the concentrations of enrofloxacin and cipmfloxacin in Chinese mitten-handed crab, Eriocheir sinensis.

关 键 词：中华绒螯蟹 反相高效液相色谱法 恩诺沙星 环丙沙星 药代动力学 

分 类 号：S948[农业科学—水产养殖]