骨髓源性神经干细胞诱导分化为多巴胺能神经元的实验研究  

Experimental study of differentiatition from Bone marrow stromal cells into dopaminergic neuron in vitro

作　　者：高健[1] 刘振华[1] 姜晓丹[2] 谢惠芳[1] 蔡颖谦[2] 邹雨汐[2] 杜谋选[2] 

机构地区：[1]南方医科大学珠江医院神经内科,广州510282 [2]南方医科大学珠江医院神经外科,广州510282

出　　处：《中华神经医学杂志》2006年第4期349-352,共4页Chinese Journal of Neuromedicine

摘　　要：目的探索骨髓基质细胞(BMSCs)诱导分化为神经干细胞及多巴胺能神经元的分化条件和发生机制,比较不同血清浓度、不同白介素-1(IL-1α)及不同胶质细胞源神经营养因子(GDNF)浓度及不同组合浓度等诱导条件下BMSCs分化情况;为BMSCs在神经科学领域内的应用奠定基础。方法以成年SD大鼠BMSCs为实验对象,利用IL-1α、胶质细胞系来源GDNF等作为增殖及分化诱导因子,进行增殖培养、分化诱导;免疫细胞法进行细胞性质鉴定。结果加入GDNF、IL-1 α增殖培养诱导6 d,部分细胞有神经巢蛋白成分表达;三周测出 DA受体D2检测阳性,GDNF+IL-1α组与 GDNF组及IL—1α组比较分化率更加显著(P<0．05)。结论 BMSCs在体外培养条件下,联合GDNF 和IL-1α诱导分化并配合使用高浓度血清(10％)可获得神经巢蛋白阳性的神经前体细胞及表达D2受体阳性的多巴胺能神经元;骨髓源性神经干细胞可以诱导分化为多巴胺能神经元。Objective To explore the feasibility and the organism of differentiation into dopaminergic neuron from bone marrow stromal cells（BMSCs） in vitro.IL-10α and different GDNF and differiating state of BMSCs induced by different consistency combination.Therefore to provide foundation for the capplication of BMSCs in the field of neurology. Methods BMSCs were acquired from adult mouse. IL-1α, glial cell line-derived neurotrophic factor （GDNF） and so on were served as proliferation and differentiation factors. Immunohistochemistry were used to identify the cells type. Results On the 6th day after adding GDNF and IL-1α into the culture medium, part of the BMSCs derived cells started to express nestin.After 3 weeks, D2 ,the receptor of DA, was tested to be positive. GDNF＋IL-1α group had more significant differentiation rate than those in GDNF group and IL-latgroup （P〈0.05）. Conclusion With the culture in vitro ,the combination with GDNF and IL-1α to induce and differentiate and serum with high consistency （10%） , BMSCs can achieve percursor cells of positive neuron nestin and dopaminergic neuron expressing D2 recptor positive.BMSCs can be induced to differentiate into dopaminergic neuron.

关 键 词：帕金森病 骨髓基质细胞 神经前体细胞 分化 多巴胺能神经元 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]