睡眠剥夺对幼鼠延髓孤束核放电、海马谷氨酸受体及其转运体表达的影响  

Sleep deprivation leads to the electric activity in NTS and the expression of NMDAR2 and GLAST in hippocampal gyrus in young rats

作　　者：王胜智[1] 王景杰[2] 马庆久[1] 何显力[1] 南菁[1] 邱建勇[3] 

机构地区：[1]西安第四军医大学唐都医院普通外科,710038 [2]西安第四军医大学唐都医院消化内科,710038 [3]第四军医大学全军神经科学研究所

出　　处：《解放军医学杂志》2006年第4期330-332,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：目的观察睡眠剥夺对幼鼠延髓内脏带(MVZ)中孤束核(NTS)电生理活动、海马中谷氨酸受体(NMDAR2)及其转运体(GLAST)的影响,为防治睡眠剥夺引起的中枢功能及学习、记忆损害提供理论依据。方法采用同步实验,应用电生理学的方法观察NTS神经元放电的变化情况,免疫组织化学的方法观察与学习、记忆密切相关的海马组织中NMDAR2及GLAST的变化情况。结果在睡眠剥夺的第3天,NTS放电明显增加,睡眠剥夺的第7天,NTS放电明显增加更为明显,而在睡眠剥夺的第2周,NTS放电呈抑制状态。在海马组织中,睡眠剥夺第3天,NMDAR2和GLAST开始增加,睡眠剥夺第5天增加更为明显,睡眠剥夺第7天,NMDAR2和GLAST增加的程度有所降低,睡眠剥夺第14天,海马中NMDAR2和GLAST表达程度与正常对照组比较无明显差异。结论睡眠剥夺对幼鼠睡眠诱导区的电生理活动有较为显著的影响。Objective To observe the effect of electric activity in the nucleus of solitary tract and the expression of NMDAR2 and GLAST in the zone of hippocaropal gyrus after sleep deprivation in younger rats, in order to provide some theoretical basis for clinical ther- apeutie strategy. IVletlmds Synchronous experiment was conducted with dectrophysiological method. The chan^es in electric-activity of nucleus of solitary tract （NTS） were determined. Immunohistochemical method was employed to detected the expression of NMDAR2 and Glast in the zone of hippocampal gyros in younger rats with sleep deprivation. Results The electric activity in nucleus of solitary tract （NTS） increased gradually along with sleep deprivation for 3 days, and the electric activity was much increased on the 7th day of sleep dep- rivatiorL In the second week of sleep deprivation, the electric activity in NTS was restrained. In the zone of hippocampal gyms, the ex- pression of both NMDAR2 and GLAST was increased significantly with 3-day sleep deprivation, and on the 5th day, the expression was much increased, while on the 7th day the expression decreased gradually. However, the expression of both MDAR2 and GLAST in the zone of hippocampal gyrus in the second week of sleep deprivation was similar to that of the control group. ~usion Sleep deprivation can exert a marked influence on the electric activity of the nucleus of solitary tract （NTS）, and may interfere with the ability of study and memory in younger rats.

关 键 词：睡眠剥夺 孤束核 电生理学 海马回 

分 类 号：R338.63[医药卫生—人体生理学]