甘宁黄土高原阿拉善黄鼠鼠疫疫源地鼠疫菌的基因分型研究  

Genomic typing of strains of Y.pestis from Spermophilus dauricua alaschanicus Plague Focus of the Loess Plateau in Gansu and Ningxia provinces

作　　者：祁美英[1] 戴二黑[2] 周冬生[2] 崔百忠[1] 金丽霞[1] 戴瑞霞[1] 赵海红[1] 李存香[1] 杨晓艳[1] 于晓涛[1] 吴得强 唐永姣 李敏[1] 杨瑞馥[2] 

机构地区：[1]青海省地方病预防控制所,西宁811602 [2]军事医学科学院微生物流行病研究所,100071 [3]甘肃省地方病预防控制所

出　　处：《中华微生物学和免疫学杂志》2006年第3期208-209,共2页Chinese Journal of Microbiology and Immunology

摘　　要：目的研究我国甘宁黄土高原阿拉善黄鼠鼠疫疫源地鼠疫菌的基因分型。方法根据已经证实的22个DFR(差异区段)设计引物,每株鼠疫菌的每个DFR都采用PCR技术进行验证。结果17株甘宁黄土高原疫源地鼠疫菌株共包括3个基因型,即7、11和13型。7、11和13型所占比例分别为5.9%(117)、5.9%(117)和88.2%(1517)。结论我国甘宁黄土高原阿拉善黄鼠鼠疫疫源地鼠疫菌的基因型主要为13型,而且13型是该疫源地鼠疫菌所特有的基因型。Objective Genomics of 17 strains of Y.pestis from Spermophilus dauricua alaxchanicus Plague Focus of the Loess Plateau in Gansu and Ningxia provinces were analyzed in order to study the genetic diversity of Y.pestis. Methods Primer pairs targeting the 22 DFRs were designed for detecting the genomic types in the strains from Spermophilus dauricua alaschanicus Plague Focus of the Loess Plateau in Gansu and Ningxia provinces. Results Three types of genomovar in 17 strains of Y. pestis were identified as genomovar 7, 11and13. The distribution of genomovar 7, 11 and 13 was 5.9%（1/17）, 5.9%（1/17） and 88.2%（15/17）, respectively. Conchusion The main and specific genomic type in strains of Y.pestis from Spermophilus dauricua alaschanicus Plague Focus of the Loess Plateau in Gansu and Ningxia provinces is genomovar 13.

关 键 词：鼠疫耶尔森菌 PCR 基因组 差异区段 自然疫源地 

分 类 号：R378[医药卫生—病原生物学]