大鼠血浆中积雪甙浓度的HPLC/MS/MS测定  

Determination of Asiaticoside in Rat Plasma by HPLC/MS/MS

作　　者：顾性初[1] 汪国权[2] 金玉娥[2] 沈朝烨[2] 刘全海[1] 

机构地区：[1]上海医药工业研究院,上海200040 [2]上海市疾病预防控制中心,上海200336

出　　处：《中国医药工业杂志》2006年第4期256-259,共4页Chinese Journal of Pharmaceuticals

摘　　要：建立了HPLC／MS／MS测定大鼠血浆中的积雪甙。以柴胡皂甙D为内标,采用XTerra C_(18)柱,流动相为0．1％乙酸和含0．1％乙酸的乙腈,梯度洗脱。质谱条件为电喷雾离子源,检测方式为多反应检测,定量分析离子为m／z981．5→493 (积雪甙)和803→331(柴胡皂甙D),血浆样品采用固相萃取处理。检测限为0．5ng／ml。A HPLC/MS/MS method was established for the determination ofasiaticoside in rat plasma. Saikosaponin D was used as the internal standard. A XTerra C18 column was used with the mobile phase of 0.1% acetic acid and acetonitrile （containing 0.1% acetic acid） by gradient elution. The electropray ionization source was used with multiple reaction monitoring mode, m/z 981.5→493 （asiaticoside） and 803→331 （saikosaponin D）. The rat plasma samples were extracted by solid phase extraction. The detection limit was 0.5ng/ml.

关 键 词：积雪甙 柴胡皂甙D 液相色谱/质谱 药物动力学 测定 

分 类 号：O657.72[理学—分析化学] O657.63[理学—化学]