活血化瘀中药对清解通下中药增效作用的实验研究Ⅱ——对急性细菌性腹膜炎大鼠肠粘膜通透性的影响  

Experimental Study on Synergistic Action of Herbs of Huoxue Huayu(活血化瘀) Herbs on Qingjie Tongxia(清解通下) HerbsⅡ—InfIuence on Intestinal Mucosal Permeability of Acute Bacterial Peritonitis in Rats

作　　者：尚晓滨[1] 吴咸中[2] 李东华[2] 郭世铎[2] 

机构地区：[1]天津医科大学,博士研究生天津300074 [2]天津市中西医结合急腹症研究所

出　　处：《中国中西医结合外科杂志》2006年第2期125-128,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：天津市科委重点支持课题(023111511)

摘　　要：目的:研究活血清下汤对急性细菌性腹膜炎大鼠肠粘膜通透性的影响。方法:采用盲肠结扎加穿孔法制作腹膜炎动物模型,将大鼠随机分为正常对照组、模型对照组、抗生素组、复方丹参方组、加味小承气汤组和活血清下汤组,每组6只动物,术后72h以乳果糖(L)、甘露醇(M)混合溶液灌胃,高效液相色谱法测定二者在尿中的含量和比值,以反映肠粘膜通透性的大小。结果:细菌性腹膜炎大鼠尿中乳果糖/甘露醇比值显著高于正常组(P<0.01),主要由于尿中乳果糖含量较高引起;各治疗组乳果糖/甘露醇比值均有所降低,尤以活血清下汤组效果显著(P<0.05或0.01)。结论:急性细菌性腹膜炎可引起肠粘膜通透性增高,活血清下汤有保护肠粘膜屏障功能的作用,使肠粘膜通透性增高受到抑制。Objective To investigate the influence of Huoxue Qingxia[HXQX] decoction on intestinal mucosal permeability in rats of acute bacterial peritonitis Methods Rat model of acute bacterial peritonitis was established with cecal ligation plus puncture（CLP）. Animals were divided into 6 groups randomly： normal group, control group and four treatment groups （Antibiotics, Fufang Danshen [ FDS ] decoction, jiawei Xiaochengqi [ JWXCQ ] decoction, and HXQX decoction）. Each group contained 6 rats. Lactulose（L） and mannitol（M） were force-fed 72 hours after operation. The amounts of L and M as well as the ratio of L/M in urine were detected by high performance liquid chromatography （HPLC） to renect the permeability of intestinal mucosa. Results The ratio of L/M was higher in rats of acute bacterial peritonitis than in normal group（p 〈 0.01）.It was resulted from higher concentration of L in urine of the former. The amounts of L and M as well as the ratio of L/M in urine were decreased to different extent in treatment groups. It was more evident in the group treated with HXQX decoction（p 〈 0.05 or 0.01）. Conclusion In acute bacterial peritonitis the permeability of intestinal mucosa is changed. HXQX decoction is of potential action on protection to permeability of intestinal mucosa and enhancement of intestinal barrier.

关 键 词：腹膜炎 肠通透性 乳果糖 甘露醇 高效液相色谱 

分 类 号：R285.5[医药卫生—中药学] Q95-3[医药卫生—中医学]