嗜热蛋白酶生产菌的筛选  

Screening of Thermophilic Proteinase Strain

作　　者：张云峰[1] 

机构地区：[1]淮阴师范学院生物学系,江苏淮安223300

出　　处：《西南师范大学学报（自然科学版）》2006年第2期157-161,共5页Journal of Southwest China Normal University(Natural Science Edition)

摘　　要：从温泉水、高温堆肥等样品中初筛到62株嗜热蛋白酶生产菌,进一步复筛出一株高产嗜热蛋白酶的生产菌株DPE7(菌株来自温泉水),该菌株被初步鉴定为芽孢杆菌(Bacillus sp．)．嗜热蛋白酶生产的最适碳源是葡萄糖, 最佳氮源为酵母粉加硫酸铵,Ca2+对菌株产酶具有促进作用．在65℃、175 r／min、pH 9的产酶培养基中振荡培养 24 h后,发酵液中的酶产量高达23．4 U／mL(60℃测定)．A high yield thermophilic proteinase strain was isolated from samples of hot spring. The strain was identified as Bacillus sp.. Suitable conditions have been studied for production of thermopbilic proteinase by Bacillus sp. DPE7, the best carbondrates is glucose, the optimum nitrogen source is yeast powder ＋ （NH4）2 SO4 and Ca^2＋ Can increase the enzyme product of the strain. The enzyme activity of supernatant is 23.4 U/mL（determined at 60 ℃） after Bacillus sp. was grown in the medium for producing proteinase for 24 h at 65 ℃, 175 r/min.

关 键 词：芽孢杆菌 嗜热蛋白酶 产酶条件 

分 类 号：Q946[生物学—植物学]