Foxp3基因转染小鼠CD4^+ CD25^- T细胞对同种T细胞增殖反应的影响  

Influence of Foxp3 gene-transferred CD4^+ CD25^- T cells on proliferation of allo T cells

作　　者：肖亚[1] 张艮甫[1] 黄赤兵[1] 贺伟峰[2] 王平贤[1] 贾维胜[1] 

机构地区：[1]第三军医大学新桥医院泌尿外科全军肾脏病中心,重庆400037 [2]第三军医大学西南医院全军烧伤研究所重庆市器官移植基础研究所,重庆400038

出　　处：《重庆医学》2006年第7期601-603,共3页Chongqing medicine

基　　金：国家自然科学基金资助(30571863)

摘　　要：目的观察Foxp3基因转染的小鼠CD4+CD25-T细胞对同种T细胞增殖反应的影响。方法以免疫磁珠法分离小鼠脾细胞中的CD4+CD25+和CD4+CD25-T细胞,用流式细胞术检测细胞纯度,以电穿孔法将质粒PMFOXP3-IRES2-EGFP转染小鼠CD4+CD25-T细胞,用荧光显微镜观察转染细胞中Foxp3的表达,转染细胞和CD4+CD25+T细胞与同种T细胞做混合淋巴细胞培养,以3H-TdR掺入测定CPM值。结果在质粒PMFOXP3-IRES2-EGFP转染CD4+CD25-T细胞24h后,转染细胞中可检测到高水平的Foxp3;转染细胞和CD4+CD25+T细胞CPM值分别为3 927.0±674.7和3 895.3±504.6(P>0.05)。结论以质粒PMFOXP3-IRES2-EGFP转染的CD4+CD25-T细胞对同种T细胞增殖反应具有显著抑制作用,与CD4+CD25+T细胞的抑制作用等同。Objective To observe the immunosuppressive efficacy of mice CD4^＋CD25^- T cells transfected with Foxp3 gene. Methods Mice CD4^＋CD25^- and CD4^＋CD25^＋ regulatory T cells were isolated by magnetic beads from the spleen. The expression of CD4 and CD25 on the cells was determined by means of flowcytometry. The isolated CD4^＋CD25^- T cells were transferred with plasmid PMFOXP3-IRES2-EGFP by electroporation. The expression of Foxp3 was determined with immune fluoresce nt microscopy. The function of Foxp3 gene transferred T cells was tested by MLC. Results A high expression of Foxp3 was foune in Foxp3 gene-transferred T cells 24h after transferring. The MLC was suppresed significantly by adding of Foxp3 gene-transferred T cells. Conclusion The CD4^＋CD25^- T cells transferred with plasmid PMFOXP3-IRES2-EGFP have the same immunosuppressive efficacy with CD4^＋CD25^＋ regulatory T cells.

关 键 词：调节性T细胞 电转染 混合淋巴细胞反应 FOXP3基因 

分 类 号：R392[医药卫生—免疫学]