苦参碱与3种抗肿瘤药物联合作用对KBV200耐药细胞株细胞周期的影响  

Effects of Matrine in Combination with 3 Antitumor Drug on Cell Cycle of KBV200 Cell Line

作　　者：陈鸿雁[1] 王驰[2] 舒艳[3] 陈燕[1] 吕彩凤[1] 余晓燕[1] 沈娜[1] 张潜英[1] 叶玲[1] 蒋纪恺[4] 

机构地区：[1]重庆医科大学附属第一医院,重庆市400016 [2]重庆医科大学基础医学院,重庆市400016 [3]重庆医科大学附属儿童医院,重庆市400014 [4]汕头大学医学院,汕头市515000

出　　处：《中国药房》2006年第7期500-501,共2页China Pharmacy

摘　　要：目的：探讨苦参碱分别与3种常用抗肿瘤药物长春新碱（VCR）、阿霉素（ADM）、顺铂（DDP）合用对KBV200耐药细胞株细胞周期的影响。方法：将培养后的KBV耐药细胞分为对照组、抗肿瘤药物组及其与苦参碱合用组，采用流式细胞仪检测、分析各组细胞周期的变化及凋亡情况。结果：与抗肿瘤药物单用组比较，苦参碱与VCR、ADM合用组细胞处于不同周期的细胞比例及凋亡数均无明显变化；苦参碱与DDP合用组G0～G1期细胞数下降、G2～M期细胞数升高。结论：推测苦参碱逆转KBV200细胞对VCR和ADM的耐药作用可能与细胞周期及凋亡无关；苦参碱能轻度增强DDP的细胞毒作用。OBJECTIVE： To observe the effects of matrine in conjunction with vincristine （VCR）, adriamycin （ADM） and diarnminedichloroplatinum （DDP）, respectively on the cell cycle of the KBV200 drug- resistant cell line. METHODS： The cultured KBV drug - resistant cells were divided into control group, antitumor drug groups and the combination therapy groups （marine ＋ antitumor drugs, respectively）, FCM assay was used to detect and analyze the expression of cell cycle and apoptosis of each group. RESULTS： As compared with single antitumor drugs groups, no significant change was found in the proportion of cells and cell apoptosis in the combination therapy groups （matrine ＋ VCR or matrine ＋ ADM）. But marine ＋ DDD resulted in a reduction of the G0-G1 stage cell population but an increase of G2-M stage cell population. CONCLUSIONS： It was assumed that matrine - induced reversal of KBV200 cells＇ drug - resistant to VCR and ADM may not relate to cell cycle and cell apoptosis. Matrine can slightly enhance the cytotoxic effect of DDP.

关 键 词：苦参碱 抗肿瘤药物 KBV200细胞株 细胞周期 

分 类 号：R285.5[医药卫生—中药学] R979.1[医药卫生—中医学]